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Recombinants within the tyrosine kinase region of v-abl and v-src identify a v-abl segment that confers lymphoid specificity

Mathey-Prevot, Bernard and Baltimore, David (1988) Recombinants within the tyrosine kinase region of v-abl and v-src identify a v-abl segment that confers lymphoid specificity. Molecular and Cellular Biology, 8 (1). pp. 234-240. ISSN 0270-7306.

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The v-abl and v-src oncogenes encode protein-tyrosine kinases that possess different biological properties in spite of their high degree of amino acid conservation. To correlate functional differences with structural domains of the two oncogenes, we recombined v-abl and v-src just downstream of the lysines in their ATP-binding sites, within the kinase domain. The biological activity of the chimeric genes was studied and compared with that of v-src and v-abl. The v-src/v-abl recombinant shared with v-src and v-abl the ability to transform fibroblasts. In addition, like v-abl, it transformed lymphoid cells and relieved a hematopoietic cell line of its interleukin 3 requirement. In contrast, the reciprocal construct, v-abl/v-src, was transformation defective. Lack of biological activity correlated with formation of a stable complex between the chimeric protein and two cellular proteins and with low kinase activity. We conclude that the specificity within the kinase domain determines the particular biological behavior of protein-tyrosine kinase oncogenes.

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Baltimore, David0000-0001-8723-8190
Additional Information:Copyright © 1988 by the American Society for Microbiology. Received 27 April 1987/Accepted 5 October 1987 We thank Joan Brugge for her generous gift of MAb327 antiserum and Jean Wang for anti-abl 584 antiserum. This work was supported by Public Health Service program project grant CA-38497 from the National Cancer Institute. B.M.-P. was supported by a Helen Hay Whitney fellowship.
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Deposited On:02 Jan 2007
Last Modified:02 Oct 2019 23:37

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