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The Synthesis of Ribosomes in E. coli. I. The Incorporation of C^(14)-Uracil into the Metabolic Pool and RNA

McCarthy, B. J. and Britten, R. J. (1962) The Synthesis of Ribosomes in E. coli. I. The Incorporation of C^(14)-Uracil into the Metabolic Pool and RNA. Biophysical Journal, 2 (1). pp. 35-47. ISSN 0006-3495. PMCID PMC1366387. doi:10.1016/S0006-3495(62)86839-8. https://resolver.caltech.edu/CaltechAUTHORS:20160727-134712765

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Abstract

C^(14)-uracil is rapidly incorporated by E. coli at low concentrations. Approximately half the radioactivity passes directly into RNA with very little delay. The remaining half enters a large metabolic pool and later is incorporated into RNA. The total rate of uptake (growing cells) is not greater than the requirement for uracil and cytosine for RNA synthesis. The size of the metabolic pool is not influenced measurably by the external uracil concentration. No evidence is found for the existence of a fraction of RNA which is rapidly synthesized and degraded.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1366387/PubMed CentralArticle
http://dx.doi.org/10.1016/S0006-3495(62)86839-8DOIArticle
Additional Information:© 1962 Biophysical Society. Received for publication, August 22, 1961.
Issue or Number:1
PubMed Central ID:PMC1366387
DOI:10.1016/S0006-3495(62)86839-8
Record Number:CaltechAUTHORS:20160727-134712765
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160727-134712765
Official Citation:Mccarthy, B. J., & Britten, R. J. (1962). The Synthesis of Ribosomes in E. coli: I. The Incorporation of C14-Uracil into the Metabolic Pool and RNA. Biophysical Journal, 2(1), 35–47.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69254
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:27 Jul 2016 20:55
Last Modified:11 Nov 2021 04:11

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