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The process of infection with bacteriophage φX174: XXV. Studies with bacteriophage φX174 mutants blocked in progeny replicative form DNA synthesis

Levine, Arnold J. and Sinsheimer, Robert L. (1969) The process of infection with bacteriophage φX174: XXV. Studies with bacteriophage φX174 mutants blocked in progeny replicative form DNA synthesis. Journal of Molecular Biology, 39 (3). pp. 619-639. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160728-075200081

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Abstract

φX174 amber mutants in cistron VI synthesize only the parental replicative form under non-permissive conditions. The specific infectivity (infectivity per molecule) of this RF‡ is similar to the parental RF made under permissive conditions. After infection of non-permissive cells with a cistron VI mutant, the parental RF attaches to a rapidly sedimenting component (membrane). The majority of this bound RF sediments as RFII. These results are similar to those obtained with wild type infection (Knippers & Sinsheimer, 1968) or with a cistron VI mutant under permissive conditions. φX lyticless mutants that synthesize normal quantities of progeny RF DNA in 0 or 30 μg of chloramphenicol/ml. do not produce progeny RF molecules when 150 μg of CM/ml. is added before infection. Addition of 150 μg of CM/ml. at eight minutes after infection (before single strand synthesis has begun) stops the production of cistron VI protein but allows RF replication to continue at onehalf the rate observed in 30 μg of CM/ml. Experiments with a cistron VI temperature-sensitive mutant demonstrate that the cistron VI gene product is continuously required for the synthesis of al progeny RF molecules. This protein does not appear to be needed for single stran synthesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(69)90149-1DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283669901491PublisherArticle
Additional Information:© 1968 Elsevier Ltd. Received 13 July, 1968. We acknowledge the excellent technical assistance of Miss Alma Shafer. We are indebted to Drs R. Knippers, J. Newbold and W. Salivar for freely supplying viral DNA and heavy phage. One of us (A.J.L.) was a postdoctoral fellow of the U.S. Public Health Service. This research was supported in part by grant GM-13554 from the U.S. Public Health Service.
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Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM 13554
NIH Postdoctoral FellowshipUNSPECIFIED
Issue or Number:3
Record Number:CaltechAUTHORS:20160728-075200081
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160728-075200081
Official Citation:Arnold J. Levine, Robert L. Sinsheimer, The process of infection with bacteriophage φX174: XXV. Studies with bacteriophage φX174 mutants blocked in progeny replicative form DNA synthesis, Journal of Molecular Biology, Volume 39, Issue 3, 14 February 1969, Pages 619-639, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(69)90149-1. (http://www.sciencedirect.com/science/article/pii/0022283669901491)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69267
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:28 Jul 2016 18:58
Last Modified:03 Oct 2019 10:20

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