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Separation of oligonucleotides by thin-layer electrophoresis

Grohmann, Karel and Sinsheimer, R. L. (1975) Separation of oligonucleotides by thin-layer electrophoresis. Analytical Biochemistry, 64 (1). pp. 214-220. ISSN 0003-2697. http://resolver.caltech.edu/CaltechAUTHORS:20160728-083545018

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Abstract

A method for fingerprinting of ^(32)P-labeled oligonucleotides is described. It involves separation by electrophoresis on cellulose thin layers in the first dimension, followed by chromatography on DEAE cellulose in the second dimension. The main advantages of the method are improved separation and simplicity.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0003-2697(75)90421-2DOIArticle
http://www.sciencedirect.com/science/article/pii/0003269775904212PublisherArticle
Additional Information:© 1975 Academic Press Inc. Received 22 July 1974, Accepted 9 October 1974. This work was supported by USPHS Grant GM 13554 from the National Institute of General Medical Sciences. One of the authors (K.G.) was supported by a postdoctoral research fellowship GM 55294 from USPHS, National Institute of General Medical Sciences.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM 13554
NIH Postdoctoral FellowshipGM 55294
Record Number:CaltechAUTHORS:20160728-083545018
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160728-083545018
Official Citation:Karel Grohmann, R.L. Sinsheimer, Separation of oligonucleotides by thin-layer electrophoresis, Analytical Biochemistry, Volume 64, Issue 1, March 1975, Pages 214-220, ISSN 0003-2697, http://dx.doi.org/10.1016/0003-2697(75)90421-2. (http://www.sciencedirect.com/science/article/pii/0003269775904212)
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ID Code:69272
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:28 Jul 2016 18:49
Last Modified:28 Jul 2016 18:49

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