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The process of infection with bacteriophage φX174: XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny φX174 bacteriophage

Knippers, Rolf and Razin, Aharon and Davis, Ronald and Sinsheimer, Robert L. (1969) The process of infection with bacteriophage φX174: XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny φX174 bacteriophage. Journal of Molecular Biology, 45 (2). pp. 237-263. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160728-103140883

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Abstract

Double-stranded φXDNA rings, composed of a closed complementary strand and an open viral strand, are found in φX-infected cells during the period of progeny single-stranded DNA formation. The linear viral strand components of these double-stranded circles are nicked between a deoxyguanosine (on the 3′-terminus) and a deoxycytidine (on the 5′-terminus). The 5′-terminus is not phosphorylated. Upon replication, nucleotides are added to the 3′-end of the viral strand. The 5′ end is concomitantly displaced from its complementary strand template and appears, by electron microscopy and by column chromatography, as a single-stranded, non-hydrogen-bonded, tail of a double-stranded circle. While the viral strand grows (to a maximum of twice the length of one φX genome) by addition of nucleotides to the 3′-end, an interesting reaction, of uncertain nature, also takes place at the free 5′-end of the nascent viral strand, the net result of which is that the original terminal deoxycytidine is replaced by a terminal deoxyguanosine. Our experiments indicate that the single-stranded DNA synthesis does not take place at the cell membrane where double-stranded φX DNA is formed but upon “cytoplasmic” RF‡. Viral coat protein can be shown to be associated with the single-strand-producing complexes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(69)90103-XDOIArticle
http://www.sciencedirect.com/science/article/pii/002228366990103XPublisherArticle
Additional Information:© 1969 Elsevier Ltd. Received 28 February 1969, Revised 3 July 1969. This research was supported in part by a grant, GM13554 from the U.S. Public Health Service and a postdoctoral research fellowship 1F05, TW-1348-01 to one of us (A. R.). Robert Rohwer performed the radio immunoassay of the experiment in Figure 8(b).
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM13554
NIH Postdoctoral Fellowship1F05-TW-1348-01
Issue or Number:2
Record Number:CaltechAUTHORS:20160728-103140883
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160728-103140883
Official Citation:Rolf Knippers, Aharon Razin, Ronald Davis, Robert L. Sinsheimer, The process of infection with bacteriophage φX174: XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny φX174 bacteriophage, Journal of Molecular Biology, Volume 45, Issue 2, 28 October 1969, Pages 237-246, IN3, 247-263, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(69)90103-X. (http://www.sciencedirect.com/science/article/pii/002228366990103X)
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ID Code:69281
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:28 Jul 2016 18:24
Last Modified:03 Oct 2019 10:20

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