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A Continuous Electroelution Method for the Recovery of DNA Restriction Enzyme Fragments

Lee, Amy Shiu and Sinsheimer, Robert L. (1974) A Continuous Electroelution Method for the Recovery of DNA Restriction Enzyme Fragments. Analytical Biochemistry, 60 (2). pp. 640-644. ISSN 0003-2697. doi:10.1016/0003-2697(74)90279-6.

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Bacterial restriction enzymes are deoxyribonucleases which cleave double-stranded DNA at specific sites and produce unique, high molecular-weight fragments. These enzymes provide a most powerful tool for studying the structure and function of DNA. The principal method for separation of the DNA fragments is electrophoresis through acrylamide or agarose gels. However, when fragments are to be isolated for further analysis, continuous elution from long cylindrical gels allows maximum separation and recovery.

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Additional Information:© 1974 Elsevier Inc. Received 17 December 1973, Accepted 20 February 1974. The authors thank Dr. K. Grohmann and Dr. P. Johnson for helpful discussion. This work was supported by U. S. Public Health Service Grant GM 13554.
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U.S. Public Health Service (USPHS)GM 13554
Issue or Number:2
Record Number:CaltechAUTHORS:20160728-125514059
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Official Citation:Amy Shiu Lee, Robert L. Sinsheimer, A continuous electroelution method for the recovery of DNA restriction enzyme fragments, Analytical Biochemistry, Volume 60, Issue 2, August 1974, Pages 640-644, ISSN 0003-2697, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69285
Deposited By: Ruth Sustaita
Deposited On:28 Jul 2016 20:19
Last Modified:11 Nov 2021 04:11

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