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The Process of Infection with Bacteriophage φX174: XXXII. Early Steps in the Infection Process: Attachment, Eclipse and DNA Penetration

Newbold, John E. and Sinsheimer, Robert L. (1970) The Process of Infection with Bacteriophage φX174: XXXII. Early Steps in the Infection Process: Attachment, Eclipse and DNA Penetration. Journal of Molecular Biology, 49 (1). pp. 49-66. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160728-130404013

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Abstract

Three distinct stages are demonstrated in the process whereby φX174 invades its host: 1. (1) Attachment: the phage attach to the cell in a manner that does not irreversibly alter the phage particles. Phage attachment per se does not harm the cell. 2. (2) Eclipse: the attached phage undergo a conformational change. Some of the altered phage particles spontaneously detach from the cell (in a non-infective form) while the remainder are more tightly bound to the cell. The altered phage particles detached (spontaneously or chemically) from such complexes have at least 40% of their DNA extruded from the phage coat. It is proposed that this particle is, or derives from, a direct intermediate in the penetration of the viral DNA. 3. (3) DNA penetration: a fraction of the attached, eclipsed phage particles corresponding in number to the phage-forming units complete DNA penetration. The penetrated DNA is found in the cell as RF, and the empty phage protein coat remains firmly attached to the exterior of the cell. This step is inhibited by prior irradiation of the phage with relatively high doses of ultraviolet light. Both eclipsed phage particles and empty phage protein coats may be dissociated from infected cells; some of their properties are described.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(70)90375-XDOIArticle
http://www.sciencedirect.com/science/article/pii/002228367090375XPublisherArticle
Additional Information:© 1970 Elsevier Ltd. Received 2 September 1969. We would like to acknowledge useful discussions with Clyde A. Hutchison, III, Dr L. Dumas and Dr G. K. Darby during the course of this work. One of us (J.E.N.) gratefully acknowledges the support of fellowships sponsored by the McCallum Fund and the Woodrow Wilson Foundation. This research was supported in part by grant no. GM 13554 from the U.S. Public Health Service.
Funders:
Funding AgencyGrant Number
McCallum FundUNSPECIFIED
Woodrow Wilson FoundationUNSPECIFIED
U.S. Public Health Service (USPHS)GM 13554
Issue or Number:1
Record Number:CaltechAUTHORS:20160728-130404013
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160728-130404013
Official Citation:John E. Newbold, Robert L. Sinsheimer, The process of infection with bacteriophage φX174: XXXII. Early steps in the infection process: Attachment, eclipse and DNA penetration, Journal of Molecular Biology, Volume 49, Issue 1, 14 April 1970, Pages 49-66, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(70)90375-X. (http://www.sciencedirect.com/science/article/pii/002228367090375X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69286
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:28 Jul 2016 20:30
Last Modified:03 Oct 2019 10:20

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