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Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy

Kim, Jinho and Henley, Beverley M. and Kim, Charlene H. and Lester, Henry A. and Yang, Changhuei (2016) Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy. Biomedical Optics Express, 7 (8). pp. 3097-3110. ISSN 2156-7085. PMCID PMC4986817. doi:10.1364/BOE.7.003097.

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Multi-day tracking of cells in culture systems can provide valuable information in bioscience experiments. We report the development of a cell culture imaging system, named EmSight, which incorporates multiple compact Fourier ptychographic microscopes with a standard multiwell imaging plate. The system is housed in an incubator and presently incorporates six microscopes. By using the same low magnification objective lenses as the objective and the tube lens, the EmSight is configured as a 1:1 imaging system that, providing large field-of-view (FOV) imaging onto a low-cost CMOS imaging sensor. The EmSight improves the image resolution by capturing a series of images of the sample at varying illumination angles; the instrument reconstructs a higher-resolution image by using the iterative Fourier ptychographic algorithm. In addition to providing high-resolution brightfield and phase imaging, the EmSight is also capable of fluorescence imaging at the native resolution of the objectives. We characterized the system using a phase Siemens star target, and show four-fold improved coherent resolution (synthetic NA of 0.42) and a depth of field of 0.2 mm. To conduct live, long-term dopaminergic neuron imaging, we cultured ventral midbrain from mice driving eGFP from the tyrosine hydroxylase promoter. The EmSight system tracks movements of dopaminergic neurons over a 21 day period.

Item Type:Article
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URLURL TypeDescription CentralArticle
Lester, Henry A.0000-0002-5470-5255
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2016 Optical Society of America. Received 23 Apr 2016; revised 7 Jul 2016; accepted 17 Jul 2016; published 22 Jul 2016. Funding: National Institute of Health (NIH) (R01AI6226-01); Caltech Agency Award (AMGEN.96EYES). We would like to thank Xiaoze Ou and Jaebum Chung for helpful discussions, and Daniel Martin for fabrication of a Siemens star phase target. Jinho Kim’s work was supported by the Kwanjeong Educational Foundation.
Funding AgencyGrant Number
Kwanjeong Educational FoundationUNSPECIFIED
Issue or Number:8
Classification Code:OCIS codes: (110.0180) Microscopy; (170.0110) Imaging systems; (170.3880) Medical and biological imaging
PubMed Central ID:PMC4986817
Record Number:CaltechAUTHORS:20160801-135931634
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Official Citation:J. Kim, B. Henley, C. Kim, H. Lester, and C. Yang, "Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy," Biomed. Opt. Express 7, 3097-3110 (2016)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69359
Deposited By: Tony Diaz
Deposited On:01 Aug 2016 22:06
Last Modified:11 Nov 2021 04:12

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