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Production and electrical characterization of the reflectin A2 isoform from Doryteuthis (Loligo) pealeii

Ordinario, David D. and Phan, Long and Walkup, Ward G., IV and Van Dyke, Yegor and Leung, Erica M. and Nguyen, Michael and Smith, Amanda G. and Kerr, Justin and Naeim, Mahan and Kymissis, Ioannis and Gorodetsky, Alon A. (2016) Production and electrical characterization of the reflectin A2 isoform from Doryteuthis (Loligo) pealeii. RSC Advances, 6 (62). pp. 57103-57107. ISSN 2046-2069. https://resolver.caltech.edu/CaltechAUTHORS:20160801-143952857

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Abstract

Cephalopods have recently emerged as a source of inspiration for the development of novel functional materials. Within this context, a number of studies have explored structural proteins known as reflectins, which play a key role in cephalopod adaptive coloration in vivo and exhibit interesting properties in vitro. Herein, we report an improved high-yield strategy for the preparation and isolation of reflectins in quantities sufficient for materials applications. We first select the Doryteuthis (Loligo) pealeii reflectin A2 (RfA2) isoform as a “model” system and validate our approach for the expression and purification of this protein. We in turn fabricate RfA2-based two-terminal devices and employ both direct and alternating current measurements to demonstrate that RfA2 films conduct protons. Our findings underscore the potential of reflectins as functional materials and may allow a wider range of researchers to investigate their properties.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1039/C6RA05405FDOIArticle
http://pubs.rsc.org/en/Content/ArticleLanding/2016/RA/C6RA05405FPublisherArticle
ORCID:
AuthorORCID
Walkup, Ward G., IV0000-0002-0385-6256
Gorodetsky, Alon A.0000-0002-3811-552X
Additional Information:© 2016 Royal Society of Chemistry. Received 29th February 2016. Accepted 28th May 2016. We thank Professor Albert Yee's Laboratory for the use of their optical microscope. We are grateful to the Air Force Office of Scientific Research (FA9550-14-1-0144) for its Financial support. W. G. W. IV acknowledges the National Science Foundation Postdoctoral Research Fellowship in Biology (DBI1306188) for its financial support.
Funders:
Funding AgencyGrant Number
Air Force Office of Scientific Research (AFOSR)FA9550-14-1-0144
NSF Postdoctoral FellowshipDBI1306188
Issue or Number:62
Record Number:CaltechAUTHORS:20160801-143952857
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160801-143952857
Official Citation:Production and electrical characterization of the reflectin A2 isoform from Doryteuthis (Loligo) pealeii David D. Ordinario, Long Phan, Ward G. Walkup IV, Yegor Van Dyke, Erica M. Leung, Michael Nguyen, Amanda G. Smith, Justin Kerr, Mahan Naeim, Ioannis Kymissis and Alon A. Gorodetsky RSC Adv., 2016, 6, 57103-57107 DOI: 10.1039/C6RA05405F
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69363
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:01 Aug 2016 22:00
Last Modified:03 Oct 2019 10:21

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