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Parallel Picoliter RT-PCR Assays Using Microfluidics

Marcus, Joshua S. and Anderson, W. French and Quake, Stephen R. (2006) Parallel Picoliter RT-PCR Assays Using Microfluidics. Analytical Chemistry, 78 (3). pp. 956-958. ISSN 0003-2700. https://resolver.caltech.edu/CaltechAUTHORS:20160802-102718412

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Abstract

The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel single cell gene expression analysis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ac0513865DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ac0513865PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ac0513865PublisherSupporting Information
Additional Information:© 2006 American Chemical Society. Received for review August 3, 2005. Accepted November 24, 2005. Publication Date (Web): January 4, 2006. The authors thank Kathy Burke, Sebastian Maerkl, and Carl Hansen for helpful discussions. This work was supported in part by a National Research Service Award (T32GM07616) from the National Institute of General Medical Sciences (J.S.M.) and National Institutes of Health (NIH) Grant NIH 1RO1 HG002644-01A1.
Funders:
Funding AgencyGrant Number
NIH Predoctoral FellowshipT32GM07616
NIH1RO1 HG002644-01A1
Issue or Number:3
Record Number:CaltechAUTHORS:20160802-102718412
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160802-102718412
Official Citation:Parallel Picoliter RT-PCR Assays Using Microfluidics Joshua S. Marcus, W. French Anderson, and Stephen R. Quake Analytical Chemistry 2006 78 (3), 956-958 DOI: 10.1021/ac0513865
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69384
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:02 Aug 2016 23:57
Last Modified:03 Oct 2019 10:21

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