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Preparation of multilamellar vesicles of defined size-distribution by solvent-spherule evaporation

Kim, Sinil and Jacobs, Russell E. and White, Stephen H. (1985) Preparation of multilamellar vesicles of defined size-distribution by solvent-spherule evaporation. Biochimica et Biophysica Acta - Biomembranes, 812 (3). pp. 793-801. ISSN 0005-2736.

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A novel method of preparing multilamellar vesicles is described. The process involves dispersing in aqueous solutions small spherules of volatile hydrophobic solvents in which amphiphathic lipids are dissolved. The lipids form vesicles when the solvents are evaporated in the proper manner. The resulting vesicles have been characterized morphologically with light microscopy and electron microscopy. The method yields multilamellar vesicles with a defined size distribution which can be adjusted by varying the duration of mechanical agitation of the spherules and by varying the concentration of amphipatic lipids in the solvents. This is the first fundamentally new method of multilamellar vesicle preparation since Bangham's report in 1965 (Bangham, A.D., Standish M.M. and Watkins J.C. (1965) J. Mol. Biol. 13, 238–252).

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Jacobs, Russell E.0000-0002-1382-8486
Additional Information:© 1985 Elsevier Science Publishers B.V. Received October 2nd, 1984. We thank Juta Kiethe for her help with the electron microscopy. This work was supported by grants from the National Science Foundation and from the National Institute of General Medical Sciences.
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Subject Keywords:Multilamellar vesicle; Liposome preparation; Size distribution; Solvent-spherule evaporation
Issue or Number:3
Record Number:CaltechAUTHORS:20160802-103357821
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Official Citation:Sinil Kim, Russell E. Jacobs, Stephen H. White, Preparation of multilamellar vesicles of defined size-distribution by solvent-spherule evaporation, Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 812, Issue 3, 14 February 1985, Pages 793-801, ISSN 0005-2736, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69385
Deposited By: Ruth Sustaita
Deposited On:03 Aug 2016 17:03
Last Modified:03 Oct 2019 10:21

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