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The process of infection with bacteriophage φX174. XXVI. Transfer of the parental DNA of bacteriophage φX174 into progeny bacteriophage particles

Knippers, Rolf and Salivar, William O. and Newbold, John E. and Sinsheimer, Robert L. (1969) The process of infection with bacteriophage φX174. XXVI. Transfer of the parental DNA of bacteriophage φX174 into progeny bacteriophage particles. Journal of Molecular Biology, 39 (3). pp. 641-654. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160802-114849063

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Abstract

The experiments described in this paper show the following: (1) Parental DNA of bacteriophage φX174 appears as an intact molecule in a newly synthesized protein coat among the progeny phage particles. (2) This transferred parental DNA has to pass through a double-stranded state, the parental replicative form DNA, before it is released into progeny particles. (3) Cytoplasmic parental replicative form DNA rather than the membrane-associated parental replicative form DNA is the precursor for the transferred DNA. (4) Single-stranded φX DNA free of coat protein can be detected in the host cell when the infection has been started in the absence of DNA synthesis. Normally, the parental single-stranded virus DNA is rapidly converted to a double-stranded replicative form DNA. (5) The super-infection exclusion phenomenon in φX-infected cells is probably based on alterations in the host cell surface.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(69)90150-8DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283669901508PublisherArticle
Additional Information:© 1969 Elsevier Ltd. Received 16 July 1968. This work was supported by grant GM 13554 from the U.S. Public Health Service.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM 13554
Issue or Number:3
Record Number:CaltechAUTHORS:20160802-114849063
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160802-114849063
Official Citation:Rolf Knippers, William O. Salivar, John E. Newbold, Robert L. Sinsheimer, The process of infection with bacteriophage φX174: XXVI. Transfer of the parental DNA of bacteriophage φX174 into progeny bacteriophage particles, Journal of Molecular Biology, Volume 39, Issue 3, 14 February 1969, Pages 641-654, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(69)90150-8. (http://www.sciencedirect.com/science/article/pii/0022283669901508)
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ID Code:69390
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:03 Aug 2016 16:59
Last Modified:03 Oct 2019 10:21

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