Sedat, John and Lyon, Alexander and Sinsheimer, Robert L. (1969) Purification of Escherichia coli pulse-labeled RNA by benzoylated DEAE-cellulose chromatography. Journal of Molecular Biology, 44 (3). pp. 415-434. ISSN 0022-2836. doi:10.1016/0022-2836(69)90370-2. https://resolver.caltech.edu/CaltechAUTHORS:20160802-121819629
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Abstract
The rapidly-labeled RNA fraction of Escherichia coli has been purified approximately 15-fold on benzoylated DEAE-cellulose columns. It is metabolically unstable (as shown by a pulse/chase experiment), and is considered to represent messenger RNA. The yield of pulse-labeled RNA is about 70% and comprises 4 to 5% of the RNA of the cell. The true size distribution of this RNA, determined by sedimentation in a denaturing solvent (99% dimethyl sulfoxide), does not change during purification. This result indicates that neither degradation nor selection for molecules of a particular size has occurred. Upon sedimentation of the final preparation in dimethyl sulfoxide, the distribution of pulse label is the same as that of RNA mass, indicating nearly complete separation from longer-lived RNA components.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1969 Elsevier Ltd. Received 31 March 1969. This research was supported in part by a grant GM13554 from the U.S. Public Health. Service. | |||||||||
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| Issue or Number: | 3 | |||||||||
| DOI: | 10.1016/0022-2836(69)90370-2 | |||||||||
| Record Number: | CaltechAUTHORS:20160802-121819629 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160802-121819629 | |||||||||
| Official Citation: | John Sedat, Alexander Lyon, Robert L. Sinsheimer, Purification of Escherichia coli pulse-labeled RNA by benzoylated DEAE-cellulose chromatography, Journal of Molecular Biology, Volume 44, Issue 3, 28 September 1969, Pages 415-434, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(69)90370-2. (http://www.sciencedirect.com/science/article/pii/0022283669903702) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 69392 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Ruth Sustaita | |||||||||
| Deposited On: | 03 Aug 2016 16:56 | |||||||||
| Last Modified: | 11 Nov 2021 04:13 |
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