Anderson, David M. and Galau, Glenn A. and Britten, Roy J. and Davidson, Eric H. (1976) Sequence complexity of the RNA accumulated in Oocytes of Arbacia punctulata. Developmental Biology, 51 (1). pp. 138-145. ISSN 0012-1606. doi:10.1016/0012-1606(76)90128-7. https://resolver.caltech.edu/CaltechAUTHORS:20160802-140947033
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Abstract
The sequence complexity of the RNA present in mature oocytes of the sea urchin Arbacia punctulata was measured by DNA-RNA hybridization. Small quantities of single copy [^3H]DNA were hybridized with large excesses of RNA and the amount of [^3H]DNA-RNA hybrids formed was assayed by hydroxyapatite. At termination of the reaction, which follows pseudo-first-order kinetics, 2.6% of the reactable single copy [^3H]DNA mass is present in RNA-DNA hybrids. This DNA was isolated, shown to be rehybridizable to RNA and, within the range of detectability, to consist solely of nonrepetitive sequences. Assuming asymmetric transcription, 5.2% of the genomic single copy sequence, or 3.0 × 10^7 nucleotides, is represented in oocyte RNA. The kinetics of hybridization indicate that these sequences are included in approximately 1–2% of the oocyte RNA.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1976 Academic Press, Inc. Accepted February 16, 1976. We would like to thank Dr. Ken Kleene of the University of Hawaii for collaboration in the preparation of Arbacia single copy DNA and Drs. Dale Graham and L. Dennis Smith for many helpful discussions. This research was supported by National Institutes of Health Training Grant HD00014 to the Marine Biological Laboratory Embryology Course, with which the authors were associated during most of the experimental work described. | |||||||||
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| Issue or Number: | 1 | |||||||||
| DOI: | 10.1016/0012-1606(76)90128-7 | |||||||||
| Record Number: | CaltechAUTHORS:20160802-140947033 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160802-140947033 | |||||||||
| Official Citation: | David M. Anderson, Glenn A. Galau, Roy J. Britten, Eric H. Davidson, Sequence complexity of the RNA accumulated in oocytes of Arbacia punctulata, Developmental Biology, Volume 51, Issue 1, 1 July 1976, Pages 138-145, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(76)90128-7. (http://www.sciencedirect.com/science/article/pii/0012160676901287) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 69394 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Ruth Sustaita | |||||||||
| Deposited On: | 02 Aug 2016 23:52 | |||||||||
| Last Modified: | 11 Nov 2021 04:13 |
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