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Sequence complexity of the RNA accumulated in Oocytes of Arbacia punctulata

Anderson, David M. and Galau, Glenn A. and Britten, Roy J. and Davidson, Eric H. (1976) Sequence complexity of the RNA accumulated in Oocytes of Arbacia punctulata. Developmental Biology, 51 (1). pp. 138-145. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160802-140947033

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Abstract

The sequence complexity of the RNA present in mature oocytes of the sea urchin Arbacia punctulata was measured by DNA-RNA hybridization. Small quantities of single copy [^3H]DNA were hybridized with large excesses of RNA and the amount of [^3H]DNA-RNA hybrids formed was assayed by hydroxyapatite. At termination of the reaction, which follows pseudo-first-order kinetics, 2.6% of the reactable single copy [^3H]DNA mass is present in RNA-DNA hybrids. This DNA was isolated, shown to be rehybridizable to RNA and, within the range of detectability, to consist solely of nonrepetitive sequences. Assuming asymmetric transcription, 5.2% of the genomic single copy sequence, or 3.0 × 10^7 nucleotides, is represented in oocyte RNA. The kinetics of hybridization indicate that these sequences are included in approximately 1–2% of the oocyte RNA.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(76)90128-7DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160676901287PublisherArticle
Additional Information:© 1976 Academic Press, Inc. Accepted February 16, 1976. We would like to thank Dr. Ken Kleene of the University of Hawaii for collaboration in the preparation of Arbacia single copy DNA and Drs. Dale Graham and L. Dennis Smith for many helpful discussions. This research was supported by National Institutes of Health Training Grant HD00014 to the Marine Biological Laboratory Embryology Course, with which the authors were associated during most of the experimental work described.
Funders:
Funding AgencyGrant Number
NIH Predoctoral FellowshipHD00014
Issue or Number:1
Record Number:CaltechAUTHORS:20160802-140947033
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160802-140947033
Official Citation:David M. Anderson, Glenn A. Galau, Roy J. Britten, Eric H. Davidson, Sequence complexity of the RNA accumulated in oocytes of Arbacia punctulata, Developmental Biology, Volume 51, Issue 1, 1 July 1976, Pages 138-145, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(76)90128-7. (http://www.sciencedirect.com/science/article/pii/0012160676901287)
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ID Code:69394
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:02 Aug 2016 23:52
Last Modified:03 Oct 2019 10:21

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