Greenlee, Lorance L. and Sinsheimer, Robert L. (1968) The process of infection with bacteriophage φX174. XVIII. Intracellular Antiserum-precipitable Components. Journal of Molecular Biology, 32 (2). pp. 321-326. ISSN 0022-2836. doi:10.1016/0022-2836(68)90012-0. https://resolver.caltech.edu/CaltechAUTHORS:20160802-142741728
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Abstract
Components precipitable by antiserum to bacteriophage φX174 can be derived from φX-infected cells. Antiserum-precipitable ^(35)S is found in three peaks after zone sedimentation in sucrose gradients. The fastest sedimenting peak is infective virus; the intermediate peak is the non-infective 70 s component. The slowest component was studied in greater detail and has the following properties. It has a sedimentation coefficient of about 6; in a thymine-requiring host, the material accumulates in the absence of thymidine; the accumulated material is subsequently incorporated into virus when thymidine is restored. The 6 s material is, therefore, considered to be a subunit of the virus structure. The φX double mutant am3tsγ, which does not make whole virus under restrictive conditions, forms a subunit which appears to have similar sedimentation properties to that of the wild type.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1968 Elsevier Ltd. Received 4 August 1967, and in revised form 6 November 1967. We wish to thank Dr Stanley Krane for helpful assistance during the initial stage of these experiments, and for making a generous supply of φX antiserum available. This research was supported in part by grants RG6965 and GM13554 from the U.S. Public Health Service. One of the authors (L.L.G.) was a post-doctoral research fellow from 1963 to 1965. | |||||||||
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| Issue or Number: | 2 | |||||||||
| DOI: | 10.1016/0022-2836(68)90012-0 | |||||||||
| Record Number: | CaltechAUTHORS:20160802-142741728 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160802-142741728 | |||||||||
| Official Citation: | Lorance L. Greenlee, Robert L. Sinsheimer, The process of infection with bacteriophage φX174: XVIII. Intracellular antiserum-precipitable components, Journal of Molecular Biology, Volume 32, Issue 2, 14 March 1968, Pages 321-326, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(68)90012-0. (http://www.sciencedirect.com/science/article/pii/0022283668900120) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 69395 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Ruth Sustaita | |||||||||
| Deposited On: | 02 Aug 2016 23:50 | |||||||||
| Last Modified: | 11 Nov 2021 04:13 |
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