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The process of infection with bacteriophage φX174. XVIII. Intracellular Antiserum-precipitable Components

Greenlee, Lorance L. and Sinsheimer, Robert L. (1968) The process of infection with bacteriophage φX174. XVIII. Intracellular Antiserum-precipitable Components. Journal of Molecular Biology, 32 (2). pp. 321-326. ISSN 0022-2836. doi:10.1016/0022-2836(68)90012-0.

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Components precipitable by antiserum to bacteriophage φX174 can be derived from φX-infected cells. Antiserum-precipitable ^(35)S is found in three peaks after zone sedimentation in sucrose gradients. The fastest sedimenting peak is infective virus; the intermediate peak is the non-infective 70 s component. The slowest component was studied in greater detail and has the following properties. It has a sedimentation coefficient of about 6; in a thymine-requiring host, the material accumulates in the absence of thymidine; the accumulated material is subsequently incorporated into virus when thymidine is restored. The 6 s material is, therefore, considered to be a subunit of the virus structure. The φX double mutant am3tsγ, which does not make whole virus under restrictive conditions, forms a subunit which appears to have similar sedimentation properties to that of the wild type.

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Additional Information:© 1968 Elsevier Ltd. Received 4 August 1967, and in revised form 6 November 1967. We wish to thank Dr Stanley Krane for helpful assistance during the initial stage of these experiments, and for making a generous supply of φX antiserum available. This research was supported in part by grants RG6965 and GM13554 from the U.S. Public Health Service. One of the authors (L.L.G.) was a post-doctoral research fellow from 1963 to 1965.
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)RG6965
U.S. Public Health Service (USPHS)GM13554
Issue or Number:2
Record Number:CaltechAUTHORS:20160802-142741728
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Official Citation:Lorance L. Greenlee, Robert L. Sinsheimer, The process of infection with bacteriophage φX174: XVIII. Intracellular antiserum-precipitable components, Journal of Molecular Biology, Volume 32, Issue 2, 14 March 1968, Pages 321-326, ISSN 0022-2836, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69395
Deposited By: Ruth Sustaita
Deposited On:02 Aug 2016 23:50
Last Modified:11 Nov 2021 04:13

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