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The structure of the DNA of bacteriophage φX174 VI. Pyrimidine sequences in the complementary strand of the replicative form

Darby, Graham and Dumas, Lawrence B. and Sinsheimer, Robert L. (1970) The structure of the DNA of bacteriophage φX174 VI. Pyrimidine sequences in the complementary strand of the replicative form. Journal of Molecular Biology, 52 (2). pp. 227-238. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160802-150838213

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Abstract

Partially synthetic duplex circles of φX174am3 DNA, specifically labeled in the pyrimidines of the complementary strand, were prepared in vitro using Escherichia coli DNA polymerase and a template of φX DNA. This material was degraded to pyrimidine tracts which were dephosphorylated and separated into fractions of uniform length by chromatography on DEAE-Sephadex. Chain lengths were assigned to the various isopliths by comparison with the profile obtained from labeled viral DNA tracts. By a combination of column Chromatographic and mapping techniques, a complete catalog of the pyrimidine tracts in the complementary strand of φX replicative form DNA (and thus, by complementarity, of the purine tracts of φX viral DNA) has been compiled.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(70)90027-6DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283670900276PublisherArticle
Additional Information:© 1970 Elsevier Ltd. Received 23 March 1970. This research was supported in part by a grant GM 13554 from the U.S. Public Health Service. One of us (L. B. D.) was supported by a postdoctoral fellowship 5-F02-AI28284 from the U.S. Public Health Service.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM 13554
NIH Postdoctoral Fellowship5-FO2-AI28284
Issue or Number:2
Record Number:CaltechAUTHORS:20160802-150838213
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160802-150838213
Official Citation:Graham Darby, Lawrence B. Dumas, Robert L. Sinsheimer, The structure of the DNA of bacteriophage φX174: VI. Pyrimidine sequences in the complementary strand of the replicative form, Journal of Molecular Biology, Volume 52, Issue 2, 14 September 1970, Pages 227-238, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(70)90027-6. (http://www.sciencedirect.com/science/article/pii/0022283670900276)
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ID Code:69400
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:02 Aug 2016 23:47
Last Modified:03 Oct 2019 10:21

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