CaltechAUTHORS
  A Caltech Library Service

Repetitive and non-repetitive sequence in sea urchin heterogeneous nuclear RNA

Smith, Michael J. and Hough, Barbara R. and Chamberlin, Margaret E. and Davidson, Eric H. (1974) Repetitive and non-repetitive sequence in sea urchin heterogeneous nuclear RNA. Journal of Molecular Biology, 85 (1). pp. 103-126. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160803-080516778

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160803-080516778

Abstract

Heterogeneous nuclear RNA was extracted from sea urchin gastrulae following a ten-minute pulse with [^3H]uridine. Hybridization reactions with excess sheared DNA were carried out using several methods. The fraction of the Hn[^3H]RNA in hybrids was determined by RNAase resistance and hydroxyapatite chromatography in a urea-phosphate medium. After incubation to a DNA C_0t value of 40, 20 to 25% of the Hn[^3H]RNA binds to hydroxyapatite. Approximately one third of these bound molecules are in repetitive DNA-RNA hybrids. The size of the bound RNA molecules measured after hybridization averages 1100 nucleotides. Up to 70% of the Hn[^3H]RNA forms hybrids at high DNA/RNA ratios (130,000/1) when reactions are carried out to high C0t values. Studies of the effect of increased DNA/RNA ratio on extent of hybridization at high DNA C_0t values show that at least two frequency classes of single copy transcript are present in the Hn[3H]RNA population. Both repetitive and single copy DNA transcripts are present within individual Hn[^3H]RNA molecules. Calculations indicate that at least 25% and possibly all of the Hn[^3H]RNA molecules contain repetitive sequence elements interspersed with non-repetitive sequences.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(74)90132-6DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283674901326PublisherArticle
Additional Information:© 1974 Elsevier Ltd. Received 4 September 1973. This research was supported by the National Institute of Child Health and Development grant no. HD05753, National Science Foundation grant no. GM33441X. One of us (M.J.S.) was a National Institute of General Medical Sciences Special Research Fellow during the course of this research. The authors express their gratitude to Dr Roy J. Britten for his critical assessment of this work and many consultations during its progress.
Funders:
Funding AgencyGrant Number
NIHHD05753
NIHGM33441X
Subject Keywords:Michael J. Smith, Barbara R. Hough, Margaret E. Chamberlin, Eric H. Davidson, Repetitive and non-repetitive sequence in sea urchin heterogeneous nuclear RNA, Journal of Molecular Biology, Volume 85, Issue 1, 5 May 1974, Pages 103-126, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(74)90132-6. (http://www.sciencedirect.com/science/article/pii/0022283674901326)
Issue or Number:1
Record Number:CaltechAUTHORS:20160803-080516778
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160803-080516778
Official Citation:Michael J. Smith, Barbara R. Hough, Margaret E. Chamberlin, Eric H. Davidson, Repetitive and non-repetitive sequence in sea urchin heterogeneous nuclear RNA, Journal of Molecular Biology, Volume 85, Issue 1, 5 May 1974, Pages 103-126, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(74)90132-6. (http://www.sciencedirect.com/science/article/pii/0022283674901326)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69402
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:03 Aug 2016 16:41
Last Modified:03 Oct 2019 10:21

Repository Staff Only: item control page