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Improvement in the Apparent Mass Resolution of Oligonucleotides by Using ^(12)C/^(14)N-Enriched Samples

Tang, Kai and Shahgholi, Mona and Garcia, Benjamin A. and Heaney, Paul J. and Cantor, Charles R. and Scott, Lincoln G. and Williamson, James R. (2002) Improvement in the Apparent Mass Resolution of Oligonucleotides by Using ^(12)C/^(14)N-Enriched Samples. Analytical Chemistry, 74 (1). pp. 226-231. ISSN 0003-2700. doi:10.1021/ac010804c.

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The apparent mass resolution of oligonucleotides in time-of-flight (TOF) mass spectrometers has been examined. In a reflectron TOF instrument, where the isotopic profile can be completely resolved, the apparent resolution matches the instrument’s resolving power. In a linear TOF instrument, unresolved isotopic profiles limit the apparent resolution to much lower values than the actual instrument resolution. By using ^(12)C/^(14)N-enriched oligonucleotides, the apparent resolution can be improved significantly. The isotope enrichment method also enhances the signal-to-noise ratio.

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Additional Information:© 2002 American Chemical Society. Received 17 July 2001; accepted 25 October 2001; published online 1 December 2001; published in print 1 January 2002. We thank Dr. Norman Chiu, Dr. Dirk van den Boom, and Professor Franz Hillenkamp for discussions; Dr. Ralf Wörl and Dr. Michael Willis for advice on RNA purification for MALDI; and Prof. Catherine Fenselau for encouragement of this work. This work was supported in part by a grant from the NIH (GM-53320 to J.R.W.).
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Issue or Number:1
Record Number:CaltechAUTHORS:20160803-155304867
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Official Citation:Tang, K., Shahgholi, M., Garcia, B. A., Heaney, P. J., Cantor, C. R., Scott, L. G., & Williamson, J. R. (2002). Improvement in the Apparent Mass Resolution of Oligonucleotides by Using 12C/14N-Enriched Samples. Analytical Chemistry, 74(1), 226-231. doi:10.1021/ac010804c
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69423
Deposited By: Melissa Ray
Deposited On:03 Aug 2016 23:19
Last Modified:11 Nov 2021 04:14

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