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The in vitro transcription units of bacteriophage φX174. II. In vitro initiation sites of φX174 transcription

Smith, L. H. and Sinsheimer, R. L. (1976) The in vitro transcription units of bacteriophage φX174. II. In vitro initiation sites of φX174 transcription. Journal of Molecular Biology, 103 (4). pp. 699-710. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160804-074258390

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Abstract

Employing the 5′ proximal labeling procedure reported previously, and hybridization to Haemophilus endonuclease bacteriophage φX RF DNA fragments, we have detected three major sites of initiation of in vitro φX transcripts. These three sites have been located on the φX174 genetic map in the following regions: (1) near the 5′ end of gene A, (2) near the 5′ end of gene B, and (3) near the 5′ end of gene D.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(76)90204-7DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283676902047PublisherArticle
Additional Information:© 1976 Elsevier Ltd. Received 30 June 1975. and in revised form 15 December 1975. We are greatful to Dr R. Espejo for his gift of ^3H-labeled φX DNA and his suggestion to use it as an internal hybridization control. We also wish to thank Drs P.H. Johnson and A. S. Lee for their generous gift of Haemophilus endonuclease preparations. This research was supported in part by grant GM13554 from the National Institutes of Health. We thank the following for their comments and discussion: Drs. J. Strauss, J. Vinograd, W. Wood, H. Mitchell and H. Rohwer.
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Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM 13554
Issue or Number:4
Record Number:CaltechAUTHORS:20160804-074258390
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160804-074258390
Official Citation:L.H. Smith, R.L. Sinsheimer, The in vitro transcription units of bacteriophage φX174: II. In vitro initiation sites of φX174 transcription, Journal of Molecular Biology, Volume 103, Issue 4, 5 June 1976, Pages 699-710, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(76)90204-7. (http://www.sciencedirect.com/science/article/pii/0022283676902047)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69427
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:04 Aug 2016 21:47
Last Modified:03 Oct 2019 10:22

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