Benbow, Robert M. and Zuccarelli, Anthony J. and Sinsheimer, Robert L. (1974) A Role for Single-strand Breaks in Bacteriophage φX174 Genetic Recombination. Journal of Molecular Biology, 88 (3). pp. 629-651. ISSN 0022-2836. doi:10.1016/0022-2836(74)90414-8. https://resolver.caltech.edu/CaltechAUTHORS:20160804-075750349
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Abstract
Formation of genetic recombinants in bacteriophage φX174 is stimulated up to 50-fold in host cells carrying the recA^+ allele by subjecting the virus particles to ultraviolet irradiation before infection, or by starving the host cell for thymine during infection; in recA host strains no such increases are observed. φX174 replicative form DNA molecules formed in vivo from ultraviolet-irradiated bacteriophage consist of an intact, circular full-length viral (+) strand and a partially complete complementary (−) strand extending from the point of origin of complementary strand DNA synthesis to an ultraviolet lesion. φX174 replicative form DNA molecules formed in thymine-deficient host strains during thymine starvation have nearly complete circular viral (+) and complementary (−) strands, which contain random single-strand nicks or gaps. Correlation of these structures with the observed increases in recombination suggests that single-strand “breaks” are aggressive intermediate structures in the formation of φX174 genetic recombinants mediated by the host recA^+ gene product.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1974 Elsevier Ltd. Received 19 December 1973, and in revised form 28 May 1974. | |||||||||
| Issue or Number: | 3 | |||||||||
| DOI: | 10.1016/0022-2836(74)90414-8 | |||||||||
| Record Number: | CaltechAUTHORS:20160804-075750349 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160804-075750349 | |||||||||
| Official Citation: | Robert M. Benbow, Anthony J. Zuccarelli, Robert L. Sinsheimer, A role for single-strand breaks in bacteriophage φX174 genetic recombination, Journal of Molecular Biology, Volume 88, Issue 3, 25 September 1974, Pages 629-638, IN11-IN22, 639-651, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(74)90414-8. (http://www.sciencedirect.com/science/article/pii/0022283674904148) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 69428 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Ruth Sustaita | |||||||||
| Deposited On: | 04 Aug 2016 21:46 | |||||||||
| Last Modified: | 11 Nov 2021 04:14 |
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