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A Role for Single-strand Breaks in Bacteriophage φX174 Genetic Recombination

Benbow, Robert M. and Zuccarelli, Anthony J. and Sinsheimer, Robert L. (1974) A Role for Single-strand Breaks in Bacteriophage φX174 Genetic Recombination. Journal of Molecular Biology, 88 (3). pp. 629-651. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160804-075750349

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Abstract

Formation of genetic recombinants in bacteriophage φX174 is stimulated up to 50-fold in host cells carrying the recA^+ allele by subjecting the virus particles to ultraviolet irradiation before infection, or by starving the host cell for thymine during infection; in recA host strains no such increases are observed. φX174 replicative form DNA molecules formed in vivo from ultraviolet-irradiated bacteriophage consist of an intact, circular full-length viral (+) strand and a partially complete complementary (−) strand extending from the point of origin of complementary strand DNA synthesis to an ultraviolet lesion. φX174 replicative form DNA molecules formed in thymine-deficient host strains during thymine starvation have nearly complete circular viral (+) and complementary (−) strands, which contain random single-strand nicks or gaps. Correlation of these structures with the observed increases in recombination suggests that single-strand “breaks” are aggressive intermediate structures in the formation of φX174 genetic recombinants mediated by the host recA^+ gene product.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(74)90414-8DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283674904148PublisherArticle
Additional Information:© 1974 Elsevier Ltd. Received 19 December 1973, and in revised form 28 May 1974.
Issue or Number:3
Record Number:CaltechAUTHORS:20160804-075750349
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160804-075750349
Official Citation:Robert M. Benbow, Anthony J. Zuccarelli, Robert L. Sinsheimer, A role for single-strand breaks in bacteriophage φX174 genetic recombination, Journal of Molecular Biology, Volume 88, Issue 3, 25 September 1974, Pages 629-638, IN11-IN22, 639-651, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(74)90414-8. (http://www.sciencedirect.com/science/article/pii/0022283674904148)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69428
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:04 Aug 2016 21:46
Last Modified:03 Oct 2019 10:22

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