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The Rat Serum Albumin Gene: Analysis of Cloned Sequences

Sargent, Thomas D. and Wu, Jung-Rung and Sala-Trepat, José M. and Wallace, R. Bruce and Reyes, Antonio A. and Bonner, James (1979) The Rat Serum Albumin Gene: Analysis of Cloned Sequences. Proceedings of the National Academy of Sciences of the United States of America, 76 (7). pp. 3256-3260. ISSN 0027-8424.

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The rat serum albumin gene has been isolated from a recombinant library containing the entire rat genome cloned in the lambda phage Charon 4A. Preliminary R-loop and restriction analysis has revealed that this gene is split into at least 14 fragments (exons) by 13 intervening sequences (introns), and that it occupies a minimum of 14.5 kilobases of genomic DNA.

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Additional Information:Copyright © 1979 by the National Academy of Sciences Contributed by James F. Bonner, April 27, 1979 We thank Merrie Jo Johnson, Marie Carter, and Leila Gonzalez for excellent technical assistance, and Mark Davis, Doug Engel, Tom Maniatis, and James Posakony for their advice and comments. This work was supported in part by U.S. Public Health Service Grants GM 13262 and GM 20927. T.D.S. was supported by a predoctoral fellowship from the National Science Foundation and R.B.W. by a fellowship of the Canadian Medical Research Council. J.M.S.-T. was on leave from Laboratoire d'Enzymologie, Centre Nationale de la Recherche Scientifique, Gif-sur-Yvette, France. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:genome libraries; cDNA clones; R-loops; Southern blots
Issue or Number:7
Record Number:CaltechAUTHORS:SARpnas79
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6943
Deposited By: Archive Administrator
Deposited On:03 Jan 2007
Last Modified:02 Oct 2019 23:37

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