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A Partial Genomic DNA Clone for the alpha Subunit of the Mouse Complement Receptor Type 3 and Cellular Adhesion Molecule Mac-1

Sastre, Leandro and Roman, Janet M. and Teplow, David B. and Dreyer, William J. and Gee, Connie and Larson, Richard S. and Roberts, Thomas M. and Springer, Timothy A. (1986) A Partial Genomic DNA Clone for the alpha Subunit of the Mouse Complement Receptor Type 3 and Cellular Adhesion Molecule Mac-1. Proceedings of the National Academy of Sciences of the United States of America, 83 (15). pp. 5644-5648. ISSN 0027-8424. PMCID PMC386345. doi:10.1073/pnas.83.15.5644.

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A genomic clone coding for the alpha subunit of the mouse complement receptor type 3 and the cellular adhesion molecule Mac-1 has been isolated directly from a genomic library using synthetic oligonucleotide probes based on the amino-terminal amino acid sequence of the protein. The identity of the clone has been established by DNA sequencing and in vitro translation of hybrid-selected mRNA. The gene is present in a single copy in the murine genome. The region containing the amino-terminal exon has been sequenced. RNA gel blotting shows that the Mac-1 alpha -subunit mRNA is 6 kilobases in length. Mac-1 alpha -subunit mRNA is present in macrophages but not T lymphoma or L cells. During gamma interferon-stimulated maturation of the mouse premyelocytic cell line M1, Mac-1 alpha -subunit mRNA is induced. This corresponds with the tissue distribution of the Mac-1 alpha subunit, showing expression is regulated at least partially at the message level.

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Additional Information:© 1986 by the National Academy of Sciences Communicated by Baruj Benacerraf, April 17, 1986. The authors thank Dr. S. Horvath for the synthesis of oligonucleotide probes, Fred King and Carolyn Anderson Gaines for expert technical assistance, and Janet Casaubon for excellent manuscript preparation. This work was supported by Grants AI20316, CA31799, and AI22304 from the National Institutes of Health. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
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Subject Keywords:oligonucleotide probes; macrophage; γ interferon-stimulated premyelocyte maturatlon; mRNA; cell adhesion molecule LFA-1
Issue or Number:15
PubMed Central ID:PMC386345
Record Number:CaltechAUTHORS:SASpnas86
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6946
Deposited By: Archive Administrator
Deposited On:03 Jan 2007
Last Modified:08 Nov 2021 20:38

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