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The in vitro transcription units of bacteriophage φX174. III. Initiation with specific 5′ end oligonucleotides of in vitro φX174 RNA

Smith, L. H. and Sinsheimer, R. L. (1976) The in vitro transcription units of bacteriophage φX174. III. Initiation with specific 5′ end oligonucleotides of in vitro φX174 RNA. Journal of Molecular Biology, 103 (4). pp. 711-735. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160805-145628389

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Abstract

Four major 5′ end oligonucleotide sequences are detected in 5′ proximally labeled in vitro bacteriophage φX174 transcripts. These are correlated to the three major initiation sites previously identified by preparative hybridization and nucleotide fingerprint analysis. In addition, the 5′ end oligonucleotides of the several discrete components isolated by preparative slab gel electrophoresis, also 5′ proximally labeled, are identified. The results confirm the genetic locations of the three major transcriptive initiation sites previously postulated.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(76)90205-9DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283676902059PublisherArticle
Additional Information:© 1976 Elsevier Ltd. Received 30 June 1975, and in revised form 16 December 1976. We are grateful to Dr J.W. Sedat for his demonstration of the sequencing procedures. This research was supported in part by grant GM13554 from the National Institutes of Health.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM 13554
Issue or Number:4
Record Number:CaltechAUTHORS:20160805-145628389
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160805-145628389
Official Citation:L.H. Smith, R.L. Sinsheimer, The in vitro transcription units of bacteriophage φX174: III. Initiation with specific 5′ end oligonucleotides of in vitro φX174 RNA, Journal of Molecular Biology, Volume 103, Issue 4, 5 June 1976, Pages 711-735, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(76)90205-9. (http://www.sciencedirect.com/science/article/pii/0022283676902059)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69481
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:05 Aug 2016 23:51
Last Modified:03 Oct 2019 10:22

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