Smith, L. H. and Sinsheimer, R. L. (1976) The in vitro transcription units of bacteriophage φX174. III. Initiation with specific 5′ end oligonucleotides of in vitro φX174 RNA. Journal of Molecular Biology, 103 (4). pp. 711-735. ISSN 0022-2836. doi:10.1016/0022-2836(76)90205-9. https://resolver.caltech.edu/CaltechAUTHORS:20160805-145628389
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Abstract
Four major 5′ end oligonucleotide sequences are detected in 5′ proximally labeled in vitro bacteriophage φX174 transcripts. These are correlated to the three major initiation sites previously identified by preparative hybridization and nucleotide fingerprint analysis. In addition, the 5′ end oligonucleotides of the several discrete components isolated by preparative slab gel electrophoresis, also 5′ proximally labeled, are identified. The results confirm the genetic locations of the three major transcriptive initiation sites previously postulated.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1976 Elsevier Ltd. Received 30 June 1975, and in revised form 16 December 1976. We are grateful to Dr J.W. Sedat for his demonstration of the sequencing procedures. This research was supported in part by grant GM13554 from the National Institutes of Health. | |||||||||
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| Issue or Number: | 4 | |||||||||
| DOI: | 10.1016/0022-2836(76)90205-9 | |||||||||
| Record Number: | CaltechAUTHORS:20160805-145628389 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160805-145628389 | |||||||||
| Official Citation: | L.H. Smith, R.L. Sinsheimer, The in vitro transcription units of bacteriophage φX174: III. Initiation with specific 5′ end oligonucleotides of in vitro φX174 RNA, Journal of Molecular Biology, Volume 103, Issue 4, 5 June 1976, Pages 711-735, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(76)90205-9. (http://www.sciencedirect.com/science/article/pii/0022283676902059) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 69481 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Ruth Sustaita | |||||||||
| Deposited On: | 05 Aug 2016 23:51 | |||||||||
| Last Modified: | 11 Nov 2021 04:15 |
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