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Detection, Mapping, and Quantification of Single Walled Carbon Nanotubes in Histological Specimens with Photoacoustic Microscopy

Avti, Pramod K. and Hu, Song and Favazza, Christopher and Mikos, Antonios G. and Jansen, John A. and Shroyer, Kenneth R. and Wang, Lihong V. and Sitharaman, Balaji (2012) Detection, Mapping, and Quantification of Single Walled Carbon Nanotubes in Histological Specimens with Photoacoustic Microscopy. PLoS ONE, 7 (4). Art. No. e35064. ISSN 1932-6203. PMCID PMC3322151. http://resolver.caltech.edu/CaltechAUTHORS:20160809-110658192

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Abstract

Aims: In the present study, the efficacy of multi-scale photoacoustic microscopy (PAM) was investigated to detect, map, and quantify trace amounts [nanograms (ng) to micrograms (µg)] of SWCNTs in a variety of histological tissue specimens consisting of cancer and benign tissue biopsies (histological specimens from implanted tissue engineering scaffolds). Materials and Methods: Optical-resolution (OR) and acoustic-resolution (AR) - Photoacoustic microscopy (PAM) was employed to detect, map and quantify the SWCNTs in a variety of tissue histological specimens and compared with other optical techniques (bright-field optical microscopy, Raman microscopy, near infrared (NIR) fluorescence microscopy). Results: Both optical-resolution and acoustic-resolution PAM, allow the detection and quantification of SWCNTs in histological specimens with scalable spatial resolution and depth penetration. The noise-equivalent detection sensitivity to SWCNTs in the specimens was calculated to be as low as ∼7 pg. Image processing analysis further allowed the mapping, distribution, and quantification of the SWCNTs in the histological sections. Conclusions: The results demonstrate the potential of PAM as a promising imaging technique to detect, map, and quantify SWCNTs in histological specimens, and could complement the capabilities of current optical and electron microscopy techniques in the analysis of histological specimens containing SWCNTs.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1371/journal.pone.0035064DOIArticle
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0035064PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322151PubMed CentralArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2012 Avti et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received November 22, 2011; Accepted March 11, 2012; Published April 9, 2012. This work was sponsored by the Carol Baldwin Fund (BS), and National Institutes of Health (BS) grant (1DP2OD007394-01) and (LVW) grants R01 EB000712, EB000712A2S1, R01 EB00071207S2, R01 EB008085, R01 CA113453901, U54 CA136398, and 5P60 DK02057933. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. LVW has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work. This does not alter the authors’ adherence to all the PLoS ONE policies on sharing data and materials. The authors would like to thank Svetlana Lublinski for assistance and analysis with SCANCO image processing language (IPL) software and data collection. Author Contributions: Conceived and designed the experiments: PKA BS. Performed the experiments: PKA SH CF BS. Analyzed the data: PKA SH CF AGM JAJ KRS LVW BS. Contributed reagents/materials/analysis tools: PKA SH CF AGM JAJ KRS LVW BS. Wrote the paper: PKA SH CF KRS LVW BS.
Funders:
Funding AgencyGrant Number
Carol Baldwin FundUNSPECIFIED
NIH1DP2OD007394-01
NIHR01 EB000712
NIHEB000712A2S1
NIHR01 EB00071207S2
NIHR01 EB008085
NIHR01 CA113453901
NIHU54 CA136398
NIH5P60 DK02057933
PubMed Central ID:PMC3322151
Record Number:CaltechAUTHORS:20160809-110658192
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160809-110658192
Official Citation:Avti PK, Hu S, Favazza C, Mikos AG, Jansen JA, et al. (2012) Detection, Mapping, and Quantification of Single Walled Carbon Nanotubes in Histological Specimens with Photoacoustic Microscopy. PLoS ONE 7(4): e35064. doi:10.1371/journal.pone.0035064
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69515
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 Aug 2016 18:40
Last Modified:09 Aug 2016 18:40

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