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Effects of light scattering on optical-resolution photoacoustic microscopy

Liu, Yan and Zhang, Chi and Wang, Lihong V. (2012) Effects of light scattering on optical-resolution photoacoustic microscopy. Journal of Biomedical Optics, 17 (12). Art. No. 126014. ISSN 1083-3668. PMCID PMC3518877. doi:10.1117/1.JBO.17.12.126014.

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The imaging depth of ballistic optical imaging technologies is limited by light scattering. To study the effects of scattering on optical-resolution photoacoustic microscopy (OR-PAM), the signals were divided into target and background signals. A method to simulate the point spread function (PSF) of the PAM system considering both optical illumination and acoustic detection was proposed, then the PSF was used to calculate the contribution of each class of signal at different depths of the focal plane (z_f). How image contrast is degraded when there is a uniformly absorbing background as well as when there are small targets densely packed in the acoustic resolution cell were studied. By using the hyperboloid-focusing-based Monte Carlo method, optical focusing into a scattering medium was simulated. It was found that the lateral resolution provided by optical focusing is degraded by only 14% when z_f=1.1 transport mean free path (l’_t), compared with the case of no scattering. When z_f =1.7 l′_t, the fluence at 50 μm radial distance away from the focal point is 93% of that at the focal point, which shows optical focusing is very weak at this depth. The method to simulate the PSF of PAM can be used in the future to optimize parameters so as to improve the system performance.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Liu, Yan0000-0002-5837-4908
Zhang, Chi0000-0002-1324-2311
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2012 SPIE. Paper 12633P received Sep. 23, 2012; revised manuscript received Nov. 12, 2012; accepted for publication Nov. 16, 2012; published online Dec. 11, 2012. We would like to thank Zijian Guo, KunWang, and Lidai Wang for helpful discussions. We would also like to thank the Cloud cluster of Washington University for computing support. This work was sponsored in part by National Institutes of Health grants R01 EB008085, R01 EB000712, R01 CA134539, R01 CA157277, R01 CA159959, and U54 CA 136398. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHR01 EB008085
NIHR01 EB000712
NIHR01 CA134539
NIHR01 CA157277
NIHR01 CA159959
NIHU54 CA 136398
Subject Keywords:photoacoustic microscopy; light scattering; optical focusing; imaging depth; Monte Carlo simulation; focused ultrasonic transducer; spatial impulse response
Issue or Number:12
PubMed Central ID:PMC3518877
Record Number:CaltechAUTHORS:20160809-152945073
Persistent URL:
Official Citation:Liu Y, Zhang C, Wang LV; Effects of light scattering on optical-resolution photoacoustic microscopy. J. Biomed. Opt. 0001;17(12):126014-126014. doi:10.1117/1.JBO.17.12.126014
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69517
Deposited By: Tony Diaz
Deposited On:10 Aug 2016 18:16
Last Modified:11 Nov 2021 04:15

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