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Förster resonance energy transfer photoacoustic microscopy

Wang, Yu and Wang, Lihong V. (2012) Förster resonance energy transfer photoacoustic microscopy. Journal of Biomedical Optics, 17 (8). Art. No. 086007. ISSN 1083-3668. PMCID PMC3414237. doi:10.1117/1.JBO.17.8.086007.

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Förster, or fluorescence, resonance energy transfer (FRET) provides fluorescence signals sensitive to intra- and inter-molecular distances in the 1 to 10 nm range. Widely applied in the fluorescence imaging environment, FRET enables visualization of physicochemical processes in molecular interactions and conformations. In this paper, we report photoacoustic imaging of FRET, based on nonradiative decay that produces heat and subsequent acoustic waves. Estimates of the energy transfer efficiency by photoacoustic microscopy were compared to those obtained by fluorescence confocal microscopy. The experimental results in tissue phantoms show that photoacoustic microscopy is capable of FRET imaging with an enhanced penetration depth. Through its ability to three-dimensionally image tissue with scalable resolution, photoacoustic microscopy could be a beneficial biomedical tool to broaden the in vivo application of FRET.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Wang, Yu0000-0003-3589-9274
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2012 SPIE. Paper 12369 received Jun. 12, 2012; revised manuscript received Jul. 10, 2012; accepted for publication Jul. 12, 2012; published online Aug. 8, 2012. This work was sponsored in part by National Institutes of Health grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, and R01 CA159959. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
NIHR01 CA157277
NIHR01 CA159959
Subject Keywords:photoacoustic microscopy; photoacoustic tomography; fluorescence microscopy; fluorescence resonance energy transfer
Issue or Number:8
PubMed Central ID:PMC3414237
Record Number:CaltechAUTHORS:20160810-073715375
Persistent URL:
Official Citation:Wang Y, Wang LV; Förster resonance energy transfer photoacoustic microscopy. J. Biomed. Opt. 0001;17(8):086007-086007. doi:10.1117/1.JBO.17.8.086007
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69526
Deposited By: Tony Diaz
Deposited On:10 Aug 2016 17:41
Last Modified:11 Nov 2021 04:15

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