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The process of infection with bacteriophage φX174. XXXIX. The structure of a DNA form with restricted binding of intercalating dyes observed during synthesis of φX single-stranded DNA

Espejo, Romilio T. and Sinsheimer, Robert L. (1976) The process of infection with bacteriophage φX174. XXXIX. The structure of a DNA form with restricted binding of intercalating dyes observed during synthesis of φX single-stranded DNA. Journal of Molecular Biology, 102 (4). pp. 723-741. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160810-145558005

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Abstract

A DNA form with restricted binding of intercalating dyes (propidium iodide or ethidium bromide) has been found in bacteriophage φX-infected cells during the period of single-stranded DNA synthesis. In the electron microscope, this DNA form is seen to be a double-stranded DNA ring with two single-stranded DNA tails protruding from the same portion of the ring; it is composed of a linear φX DNA strand, longer than one φX genome, and a single-stranded ring complementary to φX DNA. Base-pairing of these two tails in partially complementary regions restricts unwinding of the double-stranded DNA ring and consequently intercalation and binding of the dyes. It is postulated that these molecules originate from a previously reported precursor of φX DNA, namely a double-stranded ring with a single-stranded tail, by branch migration.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(76)90288-6DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283676902886PublisherArticle
Additional Information:© 1976 Elsevier Ltd. Received 29 August 1975, Revised 22 December 1975. ThH authors are grateful to Dr Harurni Kasamatsu for performing part of the electroll microscopy, her valuable advice on this technique and useful discussions. We would also like to acknowledge useful discussions with Dr John Lee Compton. This research was supported by a grant from the U.S. Public Health Service, GM13554.
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Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM 13554
Issue or Number:4
Record Number:CaltechAUTHORS:20160810-145558005
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160810-145558005
Official Citation:Romilio T. Espejo, Robert L. Sinsheimer, The process of infection with bacteriophage φX174: XXXIX. The structure of a DNA form with restricted binding of intercalating dyes observed during synthesis of φX single-stranded DNA, Journal of Molecular Biology, Volume 102, Issue 4, 25 April 1976, Pages 723-741, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(76)90288-6. (http://www.sciencedirect.com/science/article/pii/0022283676902886)
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ID Code:69550
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Aug 2016 22:06
Last Modified:03 Oct 2019 10:23

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