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Acrylamide gel electrophoresis of bacteriophage Qβ: Electrophoresis of the intact virions and of the viral proteins

Strauss, Ellen Glowacki and Kaesberg, Paul (1970) Acrylamide gel electrophoresis of bacteriophage Qβ: Electrophoresis of the intact virions and of the viral proteins. Virology, 42 (2). pp. 437-452. ISSN 0042-6822. https://resolver.caltech.edu/CaltechAUTHORS:20160810-151745794

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Abstract

Bacteriophage Qβ can be separated easily from several other RNA-containing bacteriophages by electrophoresis on large-pore acrylamide gels. The mobilities of these viruses depend both upon the acrylamide and cross-linker concentrations. Qβ has a complex electrophoretic pattern due to aggregation. An electrophoretic mutant of Qβ (called QβE1) has been isolated and partially characterized. The proteins of Qβ, QβE1, R17, and G17 have been characterized by acrylamide gel electrophoresis. Two continuous SDS-containing systems have been used: an SDS-urea-phosphate gel at pH 7.6 and an SDS-urea-phosphate gel at pH 11.9. On these, the coat proteins of Qβ and R17 and also their A proteins are separable because they differ in molecular weight. The molecular weight of the A protein of Qβ is 37,600 daltons. The coat protein of Qβ and its electrophoretic mutant QβE1 are also distinguishable though they differ by only a single charged amino acid. A low pH buffer system containing formic acid and urea but lacking SDS separates the coat proteins of Qβ, QβE1, and R17 readily.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0042-6822(70)90287-4DOIArticle
http://www.sciencedirect.com/science/article/pii/0042682270902874PublisherArticle
Additional Information:© 1970 Elsevier Inc. Accepted 24 June 1970. This work was supported by the National Institutes of Health under Grants AI 01466, AI 21,942, and GM 13554. One of us (E.G.S.) was supported by a National Institutes of Health Postdoctoral Fellowship F2-GM-21,457-01.
Funders:
Funding AgencyGrant Number
NIHAI 01466
NIHAI 21,942
NIHGM13554
NIH Postdoctoral FellowshipF2-GM-21457-01
Issue or Number:2
Record Number:CaltechAUTHORS:20160810-151745794
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160810-151745794
Official Citation:Ellen Glowacki Strauss, Paul Kaesberg, Acrylamide gel electrophoresis of bacteriophage Qβ: Electrophoresis of the intact virions and of the viral proteins, Virology, Volume 42, Issue 2, October 1970, Pages 437-452, ISSN 0042-6822, http://dx.doi.org/10.1016/0042-6822(70)90287-4. (http://www.sciencedirect.com/science/article/pii/0042682270902874)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69552
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Aug 2016 23:06
Last Modified:03 Oct 2019 10:23

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