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Sequence of histone 2B of Drosophila melanogaster

Elgin, Sarah C. R. and Schilling, James and Hood, Leroy E. (1979) Sequence of histone 2B of Drosophila melanogaster. Biochemistry, 18 (25). pp. 5679-5685. ISSN 0006-2960. http://resolver.caltech.edu/CaltechAUTHORS:20160815-065034173

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Abstract

The complete sequence of histone 2B of Drosophila has been determined by using an improved Beckman sequenator. Comparing these data with those previously published by other investigators on the histone 2B of calf [Iwai, K., Hayashi, H., & Ishikawa, K. (1972) J. Biochem. (Tokyo) 72, 357-367], trout [Koostra, A., & Bailey, G. S. (1978) Biochemistry 17, 2504-2510], and Patella (a limpet) [van Heiden, P. D., Strickland, W. N., Brandt, W. F., & von Holt, C. (1979) Eur. J. Biochem. 93, 71-78], it is possible to assess the evolutionary stability of this protein. There is little conservation of sequence in the N-terminal portion of the molecule (residues 1-26 numbering according to calf H2B), while the remainder of the protein, which we designate the C-terminal portion, is highly conserved. In the region of 27-125 residues, there are 9 substitutions in the composite data among the 98 positions, 8 of them conservative. These data indicate that very different selective pressures operate on the two different portions of the H2B molecule, implying the existence of two well-defined regions. Studies on the structure of the nucleosome by others have suggested that the C-terminal portion of H2B is involved in histone-histone interactions while the N-terminal portion is a relatively free "tail" binding to DNA. The sequence data indicate that the function of the C-terminal region of H2B requires considerable sequence specificity while that of the N-terminal region does not.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bi00592a025DOIArticle
http://pubs.acs.org/doi/abs/10.1021/bi00592a025PublisherArticle
ORCID:
AuthorORCID
Hood, Leroy E.0000-0001-7158-3678
Additional Information:© 1979 American Chemical Society. Received April 30, 1979; revised manuscript received September 27, 1979. Supported by Grant PCM 75-15259 from the National Science Foundation to S.C.R.E. and Grant GM 06945 from the National Institutes of Health to L.E.H. S.C.R.E. is the recipient of a Research Career Development Award from the National Institutes of Health.
Funders:
Funding AgencyGrant Number
NSFPCM 75-15259
NIHGM 06945
Record Number:CaltechAUTHORS:20160815-065034173
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160815-065034173
Official Citation:Sequence of histone 2B of Drosophila melanogaster Sarah C. R. Elgin, James Schilling, and Leroy E. Hood Biochemistry 1979 18 (25), 5679-5685 DOI: 10.1021/bi00592a025
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69615
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:15 Aug 2016 17:49
Last Modified:15 Aug 2016 17:49

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