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Characterization of Chemosynthetic Microbial Mats Associated with Intertidal Hydrothermal Sulfur Vents in White Point, San Pedro, CA, USA

Miranda, Priscilla J. and McLain, Nathan K. and Hatzenpichler, Roland and Orphan, Victoria J. and Dillon, Jesse G. (2016) Characterization of Chemosynthetic Microbial Mats Associated with Intertidal Hydrothermal Sulfur Vents in White Point, San Pedro, CA, USA. Frontiers in Microbiology, 7 . Art. No. 1163. ISSN 1664-302X. PMCID PMC4961709. https://resolver.caltech.edu/CaltechAUTHORS:20160815-131417667

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Abstract

The shallow-sea hydrothermal vents at White Point (WP) in Palos Verdes on the southern California coast support microbial mats and provide easily accessed settings in which to study chemolithoautotrophic sulfur cycling. Previous studies have cultured sulfur-oxidizing bacteria from the WP mats; however, almost nothing is known about the in situ diversity and activity of the microorganisms in these habitats. We studied the diversity, micron-scale spatial associations and metabolic activity of the mat community via sequence analysis of 16S rRNA and aprA genes, fluorescence in situ hybridization (FISH) microscopy and sulfate reduction rate (SRR) measurements. Sequence analysis revealed a diverse group of bacteria, dominated by sulfur cycling gamma-, epsilon-, and deltaproteobacterial lineages such as Marithrix, Sulfurovum, and Desulfuromusa. FISH microscopy suggests a close physical association between sulfur-oxidizing and sulfur-reducing genotypes, while radiotracer studies showed low, but detectable, SRR. Comparative 16S rRNA gene sequence analyses indicate the WP sulfur vent microbial mat community is similar, but distinct from other hydrothermal vent communities representing a range of biotopes and lithologic settings. These findings suggest a complete biological sulfur cycle is operating in the WP mat ecosystem mediated by diverse bacterial lineages, with some similarity with deep-sea hydrothermal vent communities.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.3389/fmicb.2016.01163DOIArticle
http://journal.frontiersin.org/article/10.3389/fmicb.2016.01163/fullPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961709/PubMed CentralArticle
ORCID:
AuthorORCID
Hatzenpichler, Roland0000-0002-5489-3444
Orphan, Victoria J.0000-0002-5374-6178
Additional Information:© 2016 Miranda, McLain, Hatzenpichler, Orphan and Dillon. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Received: 01 April 2016; Accepted: 12 July 2016; Published: 27 July 2016. Author Contributions: Conception or design of the work: PM, RH, VO, and JD. Data acquisition, analysis and interpretation: PM, NM, RH, and JD. Drafting the article: PM, and JD. Critical revision of the article: PM, NM, RH, VO, and JD. All authors have read and approved this submission. This study was performed with support from the National Science Foundation (EAR-1124398 to JD, EAR-112391 to VO) and CSU-COAST student research grant to PM. Partial support was additionally provided by the Gordon and Betty Moore Foundation through grant GBMF3306 to VO. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. We thank Christine Whitcraft and Lora Stevens for project advising and Katherine Dawson for providing the WP sulfide measurements.
Funders:
Funding AgencyGrant Number
NSFEAR-1124398
NSFEAR-112391
CSU-COAST student research grantUNSPECIFIED
Gordon and Betty Moore FoundationGBMF3306
Subject Keywords:hydrothermal vents, microbial mat, Desulfuromusa, sulfur-cycling, sulfate reduction rates, pyrosequencing, fluorescence in situ hybridization (FISH)
PubMed Central ID:PMC4961709
Record Number:CaltechAUTHORS:20160815-131417667
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160815-131417667
Official Citation:Miranda PJ, McLain NK, Hatzenpichler R, Orphan VJ and Dillon JG (2016) Characterization of Chemosynthetic Microbial Mats Associated with Intertidal Hydrothermal Sulfur Vents in White Point, San Pedro, CA, USA. Front. Microbiol. 7:1163. doi: 10.3389/fmicb.2016.01163
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69624
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:15 Aug 2016 22:18
Last Modified:09 Mar 2020 13:18

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