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A Link between Integral Membrane Protein Expression and Simulated Integration Efficiency

Marshall, Stephen S. and Niesen, Michiel J. M. and Müller, Axel and Tiemann, Katrin and Saladi, Shyam M. and Galimidi, Rachel P. and Zhang, Bin and Clemons, William M., Jr. and Miller, Thomas F., III (2016) A Link between Integral Membrane Protein Expression and Simulated Integration Efficiency. Cell Reports, 16 (8). pp. 2169-2177. ISSN 2211-1247. PMCID PMC5001948. http://resolver.caltech.edu/CaltechAUTHORS:20160816-075308782

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Abstract

Integral membrane proteins (IMPs) control the flow of information and nutrients across cell membranes, yet IMP mechanistic studies are hindered by difficulties in expression. We investigate this issue by addressing the connection between IMP sequence and observed expression levels. For homologs of the IMP TatC, observed expression levels vary widely and are affected by small changes in protein sequence. The effect of sequence changes on experimentally observed expression levels strongly correlates with the simulated integration efficiency obtained from coarse-grained modeling, which is directly confirmed using an in vivo assay. Furthermore, mutations that improve the simulated integration efficiency likewise increase the experimentally observed expression levels. Demonstration of these trends in both Escherichia coli and Mycobacterium smegmatis suggests that the results are general to other expression systems. This work suggests that IMP integration is a determinant for successful expression, raising the possibility of controlling IMP expression via rational design.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.celrep.2016.07.042DOIArticle
http://www.sciencedirect.com/science/article/pii/S2211124716309603PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001948/PubMed CentralArticle
ORCID:
AuthorORCID
Marshall, Stephen S.0000-0003-2263-6854
Niesen, Michiel J. M.0000-0002-9255-6203
Clemons, William M., Jr.0000-0002-0021-889X
Miller, Thomas F., III0000-0002-1882-5380
Additional Information:© 2016 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Received 4 January 2016, Revised 9 June 2016, Accepted 16 July 2016, Available online 11 August 2016. Published: August 11, 2016. The authors thank R.C. Van Lehn and D.C. Rees for comments on the manuscript and D. Daley for helpful discussion of Daley et al. (2005). Work in the W.M.C. lab is supported by an NIH Pioneer Award to W. M. C. (5DP1GM105385) and an NIH training grant to S.S.M. (NIH/National Research Service Award (NRSA) training grant 5T32GM07616). Work in the T.F.M. group is supported in part by the Office of Naval Research (N00014-10-1-0884), and computational resources were provided by the National Energy Research Scientific Computing Center (NERSC), a (Department of Energy) DOE Office of Science User Facility (DE-AC02-05CH11231).
Group:Caltech Center for Environmental Microbial Interactions (CEMI)
Funders:
Funding AgencyGrant Number
NIH5DP1GM105385
NIH Predoctoral Fellowship5T32GM07616
Office of Naval Research (ONR)N00014-10-1-0884
National Energy Research Scientific Computing Center (NERSC)UNSPECIFIED
Department of Energy (DOE)DE-AC02-05CH11231
PubMed Central ID:PMC5001948
Record Number:CaltechAUTHORS:20160816-075308782
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160816-075308782
Official Citation:Stephen S. Marshall, Michiel J.M. Niesen, Axel Müller, Katrin Tiemann, Shyam M. Saladi, Rachel P. Galimidi, Bin Zhang, William M. Clemons Jr., Thomas F. Miller III, A Link between Integral Membrane Protein Expression and Simulated Integration Efficiency, Cell Reports, Volume 16, Issue 8, 23 August 2016, Pages 2169-2177, ISSN 2211-1247, http://dx.doi.org/10.1016/j.celrep.2016.07.042. (http://www.sciencedirect.com/science/article/pii/S2211124716309603)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69641
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:16 Aug 2016 18:19
Last Modified:01 Dec 2017 18:34

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