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Live Staining of Drosophila Embryos with RPTP Fusion Proteins to Detect and Characterize Expression of Cell-Surface RPTP Ligands

Bali, Namrata and Lee, Hyung-Kook (Peter) and Zinn, Kai (2016) Live Staining of Drosophila Embryos with RPTP Fusion Proteins to Detect and Characterize Expression of Cell-Surface RPTP Ligands. In: Protein Tyrosine Phosphatases. Methods in Molecular Biology. No.1447. Springer , New York, NY, pp. 373-384. ISBN 978-1-4939-3744-8. http://resolver.caltech.edu/CaltechAUTHORS:20160816-080034598

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Abstract

The activity and/or localization of receptor tyrosine kinases and phosphatases are controlled by binding to cell-surface or secreted ligands. Identification of ligands for receptor tyrosine phosphatases (RPTPs) is essential for understanding their in vivo functions during development and disease. Here we describe a novel in vivo method to identify ligands and binding partners for RPTPs by staining live-dissected Drosophila embryos. Live dissected embryos are incubated with RPTP fusion proteins to detect ligand binding in embryos. This method can be streamlined to perform large-scale screens for ligands as well as to search for embryonic phenotypes.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/978-1-4939-3746-2_20DOIArticle
http://link.springer.com/protocol/10.1007%2F978-1-4939-3746-2_20PublisherArticle
ORCID:
AuthorORCID
Zinn, Kai0000-0002-6706-5605
Additional Information:© 2016 Springer Science+Business Media New York.
Subject Keywords:Receptor tyrosine phosphatases; Live dissection; Fusion proteins; Ligand screening; Schneider cells
Record Number:CaltechAUTHORS:20160816-080034598
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160816-080034598
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69642
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Aug 2016 18:20
Last Modified:16 Aug 2016 18:20

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