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Time-reversed ultrasonically encoded optical focusing in biological tissue

Lai, Puxiang and Xu, Xiao and Liu, Honglin and Wang, Lihong V. (2012) Time-reversed ultrasonically encoded optical focusing in biological tissue. Journal of Biomedical Optics, 17 (3). Art. No. 030506. ISSN 1083-3668. PMCID PMC3380942; PMC4098039.

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We report an experimental investigation of time-reversed ultrasonically encoded optical focusing in biological tissue. This technology combines the concepts of optical phase conjugation and ultrasound modulation of diffused coherent light. The ultrasonically encoded (or tagged) diffused light from a tissue sample is collected in reflection mode and interferes with a reference light in a photorefractive crystal (used as a phase conjugation mirror) to form a hologram. Then a time-reversed copy of the tagged light is generated and traces back the original trajectories to the ultrasonic focus inside the tissue sample. With our current setup, we can achieve a maximum penetration depth of 5 mm in a chicken breast sample and image optical contrasts within a tissue sample with a spatial resolution approximately equaling 1/√2 of the ultrasound focal diameter.

Item Type:Article
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URLURL TypeDescription CentralArticle CentralArticle - Erratum
Liu, Honglin0000-0003-2473-9056
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2012 SPIE. Paper 11563 received Sep. 30, 2011; revised manuscript received Jan. 20, 2012; accepted for publication Jan. 23, 2012; published online Mar. 8, 2012; corrected Mar. 19, 2012. This research is sponsored in part by the National Academies Keck Futures Initiative grant IS 13 and the National Institute of Health through grants R01 EB000712 and U54 CA136398. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Errata:This article was originally published in Vol. 17 of the Journal of Biomedical Optics on 8 March 2012 in the wrong section of the table of contents and with an incorrect citation identifier (CID) of 036001. The paper was removed and republished online with the correct CID of 030506 on 19 March 2012. This change was made in order for the paper to appear under the correct section heading, “JBO Letters.” For more information on the use of CIDs in JBO, see
Funding AgencyGrant Number
National Academies Keck Futures InitiativeIS 13
NIHR01 EB000712
NIHU54 CA136398
Subject Keywords:optical imaging; optical focusing; ultrasound modulation; optical phase conjugation; time reversal; photorefractive crystal; reflection mode; biological tissue
Issue or Number:3
PubMed Central ID:PMC3380942; PMC4098039
Record Number:CaltechAUTHORS:20160816-155345267
Persistent URL:
Official Citation:Lai P, Xu X, Liu H, Wang LV; Time-reversed ultrasonically encoded optical focusing in biological tissue. J. Biomed. Opt. 0001;17(3):030506-1-030506-3. doi:10.1117/1.JBO.17.3.030506
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69683
Deposited By: Tony Diaz
Deposited On:16 Aug 2016 22:58
Last Modified:09 Mar 2020 13:19

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