Yao, Junjie and Huang, Chih-Hsien and Wang, Lidai and Yang, Joon-Mo and Gao, Liang and Maslov, Konstantin I. and Zou, Jun and Wang, Lihong V. (2012) Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror. Journal of Biomedical Optics, 17 (8). Art. No. 080505. ISSN 1083-3668. PMCID PMC3418511. https://resolver.caltech.edu/CaltechAUTHORS:20160817-084003471
![]() |
PDF
- Published Version
See Usage Policy. 1222Kb |
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160817-084003471
Abstract
By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have to sacrifice either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible microelectromechanical systems (MEMS) scanning mirror (MEMS-OR-PAM). In MEMS-OR-PAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures the uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. Presented results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.
Item Type: | Article | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Related URLs: |
| ||||||||||||||||
ORCID: |
| ||||||||||||||||
Additional Information: | © 2012 SPIE. Paper 12347L received Jun. 1, 2012; revised manuscript received Jul. 19, 2012; accepted for publication Jul. 24, 2012; published online Aug. 14, 2012. The authors appreciate Prof. James Ballard’s close reading of the manuscript. This work was sponsored by NIH grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, and R01 CA159959 (for L. V. Wang), and NSF grant CMMI-1131758 (for J. Zou). L. V. Wang has a financial interest in Endra, Inc., and Microphotoacoustics, Inc., which, however, did not support this work. K. I. Maslov has a financial interest in Microphotoacoustics, Inc., which did not support this work. | ||||||||||||||||
Funders: |
| ||||||||||||||||
Subject Keywords: | optical-resolution photoacoustic microscopy; fast scanning; MEMS scanning mirror; blood flow dynamics; confocality; confocal sensitivity | ||||||||||||||||
Issue or Number: | 8 | ||||||||||||||||
PubMed Central ID: | PMC3418511 | ||||||||||||||||
Record Number: | CaltechAUTHORS:20160817-084003471 | ||||||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160817-084003471 | ||||||||||||||||
Official Citation: | Yao J, Huang C, Wang L, et al; Wide-field fast-scanning photoacoustic microscopy based on a water-immersible mems scanning mirror. J. Biomed. Opt. 0001;17(8):080505-1-080505-3. doi:10.1117/1.JBO.17.8.080505 | ||||||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||||||
ID Code: | 69688 | ||||||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||||||
Deposited By: | Tony Diaz | ||||||||||||||||
Deposited On: | 17 Aug 2016 18:47 | ||||||||||||||||
Last Modified: | 09 Mar 2020 13:19 |
Repository Staff Only: item control page