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Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror

Yao, Junjie and Huang, Chih-Hsien and Wang, Lidai and Yang, Joon-Mo and Gao, Liang and Maslov, Konstantin I. and Zou, Jun and Wang, Lihong V. (2012) Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror. Journal of Biomedical Optics, 17 (8). Art. No. 080505. ISSN 1083-3668. PMCID PMC3418511.

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By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have to sacrifice either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible microelectromechanical systems (MEMS) scanning mirror (MEMS-OR-PAM). In MEMS-OR-PAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures the uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. Presented results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Maslov, Konstantin I.0000-0003-3408-8840
Zou, Jun0000-0002-9543-6135
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2012 SPIE. Paper 12347L received Jun. 1, 2012; revised manuscript received Jul. 19, 2012; accepted for publication Jul. 24, 2012; published online Aug. 14, 2012. The authors appreciate Prof. James Ballard’s close reading of the manuscript. This work was sponsored by NIH grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, and R01 CA159959 (for L. V. Wang), and NSF grant CMMI-1131758 (for J. Zou). L. V. Wang has a financial interest in Endra, Inc., and Microphotoacoustics, Inc., which, however, did not support this work. K. I. Maslov has a financial interest in Microphotoacoustics, Inc., which did not support this work.
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
NIHR01 CA157277
NIHR01 CA159959
Subject Keywords:optical-resolution photoacoustic microscopy; fast scanning; MEMS scanning mirror; blood flow dynamics; confocality; confocal sensitivity
Issue or Number:8
PubMed Central ID:PMC3418511
Record Number:CaltechAUTHORS:20160817-084003471
Persistent URL:
Official Citation:Yao J, Huang C, Wang L, et al; Wide-field fast-scanning photoacoustic microscopy based on a water-immersible mems scanning mirror. J. Biomed. Opt. 0001;17(8):080505-1-080505-3. doi:10.1117/1.JBO.17.8.080505
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69688
Deposited By: Tony Diaz
Deposited On:17 Aug 2016 18:47
Last Modified:09 Mar 2020 13:19

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