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Fiber-laser-based photoacoustic microscopy and melanoma cell detection

Wang, Yu and Maslov, Konstantin and Zhang, Yu and Hu, Song and Yang, Lihmei and Xia, Younan and Liu, Jian and Wang, Lihong V. (2011) Fiber-laser-based photoacoustic microscopy and melanoma cell detection. Journal of Biomedical Optics, 16 (1). Art. No. 011014. ISSN 1083-3668. PMCID PMC3033874.

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For broad applications in biomedical research involving functional dynamics and clinical studies, a photoacoustic microscopy system should be compact, stable, and fast. In this work, we use a fiber laser as the photoacoustic irradiation source to meet these goals. The laser system measures 45×56×13 cm^3. The stability of the laser is attributed to the intrinsic optical fiber-based light amplification and output coupling. Its 50-kHz pulse repetition rate enables fast scanning or extensive signal averaging. At the laser wavelength of 1064 nm, the photoacoustic microscope still has enough sensitivity to image small blood vessels while providing high optical absorption contrast between melanin and hemoglobin. Label-free melanoma cells in flowing bovine blood are imaged in vitro, yielding measurements of both cell size and flow speed.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Wang, Yu0000-0003-3589-9274
Maslov, Konstantin0000-0003-3408-8840
Zhang, Yu0000-0001-8938-1927
Liu, Jian0000-0001-8552-1400
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2011 SPIE. Paper 10158SSR received Mar. 24, 2010; revised manuscript received Sep. 23, 2010; accepted for publication Oct. 21, 2010; published online Jan. 11, 2011. This work was supported in part by National Institutes of Health Grants Nos. R01 EB000712, R01 EB008085, R01 CA113453901, U54 CA136398, 5P60 DK02057933, and R43 RR025928. Wang has a financial interest in Microphotoacoustics, Incorporated, and Endra, Incorporated, which, however, did not support this work. The authors are grateful to Christopher Favazza for beneficial discussions. They also thank Jim Ballard for careful proofreading.
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA113453901
NIHU54 CA136398
NIH5P60 DK02057933
NIHR43 RR025928
Subject Keywords:photoacoustic microscopy; fiber laser; high pulse repetition rate; vasculature imaging; circulating melanoma cells imaging; cell size; cell flow speed
Issue or Number:1
PubMed Central ID:PMC3033874
Record Number:CaltechAUTHORS:20160817-095425102
Persistent URL:
Official Citation:Wang Y, Maslov K, Zhang Y, et al; Fiber-laser-based photoacoustic microscopy and melanoma cell detection. J. Biomed. Opt. 0001;16(1):011014-011014-4. doi:10.1117/1.3525643
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69702
Deposited By: Tony Diaz
Deposited On:17 Aug 2016 18:39
Last Modified:09 Mar 2020 13:19

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