In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure

Wang, Yu and Hu, Song and Maslov, Konstantin and Zhang, Yu and Xia, Younan and Wang, Lihong V. (2011) In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure. Optics Letters, 36 (7). pp. 1029-1031. ISSN 0146-9592. PMCID PMC3076119. http://resolver.caltech.edu/CaltechAUTHORS:20160817-131445520

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Abstract

We developed dual-modality microscope integrating photoacoustic microscopy (PAM) and fluorescence confocal microscopy (FCM) to noninvasively image hemoglobin oxygen saturation (sO_2) and oxygen partial pressure (pO_2) in vivo in single blood vessels with high spatial resolution. While PAM measures sO_2 by imaging hemoglobin optical absorption at two wavelengths, FCM quantifies pO_2 using phosphorescence quenching. The variations of sO_2 and pO_2 values in multiple orders of vessel branches under hyperoxic (100% oxygen) and normoxic (21% oxygen) conditions correlate well with the oxygen–hemoglobin dissociation curve. In addition, the total concentration of hemoglobin is imaged by PAM at an isosbestic wavelength.

Item Type:

Article

Related URLs:

URL	URL Type	Description
http://dx.doi.org/10.1364/OL.36.001029	DOI	Article
https://www.osapublishing.org/ol/abstract.cfm?uri=ol-36-7-1029	Publisher	Article
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076119	PubMed Central	Article

ORCID:

Author	ORCID
Wang, Lihong V.	0000-0001-9783-4383

Additional Information:

© 2011 Optical Society of America. Received December 15, 2010; revised January 29, 2011; accepted February 18, 2011; posted February 23, 2011 (Doc. ID 139708); published March 16, 2011. We thank C. Zhang for helping with data processing and manuscript preparation. This work was sponsored in part by National Institutes of Health (NIH) grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, and 5P60 DK02057933. L. Wang has a financial interest in Microphotoacoustics, Inc., and Endra, Inc., which, however, did not support this work.

Funders:

Funding Agency	Grant Number
NIH	R01 EB000712
NIH	R01 EB008085
NIH	R01 CA134539
NIH	U54 CA136398
NIH	5P60 DK02057933

Classification Code:

OCIS codes: 170.3880, 170.5120, 170.1790, 180.5810

PubMed Central ID:

PMC3076119

Record Number:

CaltechAUTHORS:20160817-131445520

Persistent URL:

http://resolver.caltech.edu/CaltechAUTHORS:20160817-131445520

Official Citation:

Yu Wang, Song Hu, Konstantin Maslov, Yu Zhang, Younan Xia, and Lihong V. Wang, "In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure," Opt. Lett. 36, 1029-1031 (2011)

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ID Code:

69710

Collection:

CaltechAUTHORS

Deposited By:

Tony Diaz

Deposited On:

17 Aug 2016 21:41

Last Modified:

17 Aug 2016 21:41

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