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In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure

Wang, Yu and Hu, Song and Maslov, Konstantin and Zhang, Yu and Xia, Younan and Wang, Lihong V. (2011) In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure. Optics Letters, 36 (7). pp. 1029-1031. ISSN 0146-9592. PMCID PMC3076119. http://resolver.caltech.edu/CaltechAUTHORS:20160817-131445520

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Abstract

We developed dual-modality microscope integrating photoacoustic microscopy (PAM) and fluorescence confocal microscopy (FCM) to noninvasively image hemoglobin oxygen saturation (sO_2) and oxygen partial pressure (pO_2) in vivo in single blood vessels with high spatial resolution. While PAM measures sO_2 by imaging hemoglobin optical absorption at two wavelengths, FCM quantifies pO_2 using phosphorescence quenching. The variations of sO_2 and pO_2 values in multiple orders of vessel branches under hyperoxic (100% oxygen) and normoxic (21% oxygen) conditions correlate well with the oxygen–hemoglobin dissociation curve. In addition, the total concentration of hemoglobin is imaged by PAM at an isosbestic wavelength.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1364/OL.36.001029DOIArticle
https://www.osapublishing.org/ol/abstract.cfm?uri=ol-36-7-1029PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076119PubMed CentralArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2011 Optical Society of America. Received December 15, 2010; revised January 29, 2011; accepted February 18, 2011; posted February 23, 2011 (Doc. ID 139708); published March 16, 2011. We thank C. Zhang for helping with data processing and manuscript preparation. This work was sponsored in part by National Institutes of Health (NIH) grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, and 5P60 DK02057933. L. Wang has a financial interest in Microphotoacoustics, Inc., and Endra, Inc., which, however, did not support this work.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
NIH5P60 DK02057933
Classification Code:OCIS codes: 170.3880, 170.5120, 170.1790, 180.5810
PubMed Central ID:PMC3076119
Record Number:CaltechAUTHORS:20160817-131445520
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160817-131445520
Official Citation:Yu Wang, Song Hu, Konstantin Maslov, Yu Zhang, Younan Xia, and Lihong V. Wang, "In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure," Opt. Lett. 36, 1029-1031 (2011)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69710
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:17 Aug 2016 21:41
Last Modified:17 Aug 2016 21:41

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