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Enzyme functionality and solvation of Subtilisin Carlsberg: from hours to femtoseconds

Kamal, J. K. Amisha and Xia, Tianbing and Pal, Samir Kumar and Zhao, Liang and Zewail, Ahmed H. (2004) Enzyme functionality and solvation of Subtilisin Carlsberg: from hours to femtoseconds. Chemical Physics Letters, 387 (4-6). pp. 209-215. ISSN 0009-2614. doi:10.1016/j.cplett.2004.02.011. https://resolver.caltech.edu/CaltechAUTHORS:20160817-132620860

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Abstract

We report studies of the enzymatic activity of Subtilisin Carlsberg (SC) in different solvents and pH's using two substrates. From the Michaelis–Menten mechanism, we found the specificity constant (k_(cat)/K_M) of enzymatic activity to be retarded when organic solvents, such as acetonitrile or dioxane, are added to the aqueous medium or when the pH is lowered. In order to address the role of solvation, we also studied the femtosecond dynamics of the enzyme and the solubility of substrates and products. We elucidate the nature of the free energy surface from the knowledge of the free energy change (K_M), catalytic turn over (k_(cat)), solvation, and effect of pH on the enzymatic activity.


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http://dx.doi.org/10.1016/j.cplett.2004.02.011DOIArticle
http://www.sciencedirect.com/science/article/pii/S000926140400209XPublisherArticle
Additional Information:© 2004 Elsevier B.V. Received 12 January 2004, Available online 2 March 2004. This work is supported by National Science Foundation (NSF).
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Funding AgencyGrant Number
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Issue or Number:4-6
DOI:10.1016/j.cplett.2004.02.011
Record Number:CaltechAUTHORS:20160817-132620860
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160817-132620860
Official Citation:J.K.Amisha Kamal, Tianbing Xia, Samir Kumar Pal, Liang Zhao, Ahmed H Zewail, Enzyme functionality and solvation of Subtilisin Carlsberg: from hours to femtoseconds, Chemical Physics Letters, Volume 387, Issues 4–6, 1 April 2004, Pages 209-215, ISSN 0009-2614, http://dx.doi.org/10.1016/j.cplett.2004.02.011. (http://www.sciencedirect.com/science/article/pii/S000926140400209X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69711
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:22 Aug 2016 23:41
Last Modified:11 Nov 2021 04:18

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