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Label-Free Bond-Selective Imaging by Listening to Vibrationally Excited Molecules

Wang, Han-Wei and Chai, Ning and Wang, Pu and Hu, Song and Dou, Wei and Umulis, David and Wang, Lihong V. and Sturek, Michael and Lucht, Robert and Cheng, Ji-Xin (2011) Label-Free Bond-Selective Imaging by Listening to Vibrationally Excited Molecules. Physical Review Letters, 106 (23). Art. No. 238106. ISSN 0031-9007. PMCID PMC3398792. https://resolver.caltech.edu/CaltechAUTHORS:20160818-105215538

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Abstract

We report the realization of vibrational photoacoustic (VPA) microscopy using optical excitation of molecular overtone vibration and acoustic detection of the resultant pressure transients. Our approach eliminates the tissue scattering problem encountered in near-infrared spectroscopy and enables depth-resolved signal collection. The 2nd overtone of the CH bond stretch around 8300  cm^(−1), where blood interference is minimal, is excited. We demonstrate 3D VPA imaging of lipid-rich atherosclerotic plaques by excitation from the artery lumen, and lipid storage in live Drosophila larvae, with millimeter-scale penetration depth.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1103/PhysRevLett.106.238106DOIArticle
http://journals.aps.org/prl/abstract/10.1103/PhysRevLett.106.238106PublisherArticle
http://journals.aps.org/prl/supplemental/10.1103/PhysRevLett.106.238106PublisherSupplemental Material
http://dx.doi.org/10.1103/PhysRevLett.106.259901ErrataErratum
http://journals.aps.org/prl/abstract/10.1103/PhysRevLett.106.259901ErrataErratum
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398792PubMed CentralArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2011 American Physical Society. Received 12 November 2010; revised manuscript received 7 May 2011; published 10 June 2011; publisher error corrected 15 June 2011. We acknowledge a grant from the American Heart Association to H.W.W.; support from R01 EB7243 to J. X. C.; HL062552 and the Fortune-Fry Ultrasound Research Fund to M. S.; and R01 EB000712, U54 CA136398, and 5P60 DK02057933 to L.V.W.
Errata:Publisher’s Note: Label-Free Bond-Selective Imaging by Listening to Vibrationally Excited Molecules [Phys. Rev. Lett. 106, 238106 (2011)] Han-Wei Wang, Ning Chai, Pu Wang, Song Hu, Wei Dou, David Umulis, Lihong V. Wang, Michael Sturek, Robert Lucht, and Ji-Xin Cheng Phys. Rev. Lett. 106, 259901
Funders:
Funding AgencyGrant Number
American Heart AssociationUNSPECIFIED
NIHR01 EB7243
NIHHL062552
Fortune-Fry Ultrasound Research FundUNSPECIFIED
NIHR01 EB000712
NIHU54 CA136398
NIH5P60 DK02057933
Issue or Number:23
Classification Code:PACS numbers: 87.85.Pq, 34.50.Ez, 42.62.Be, 78.20.Pa
PubMed Central ID:PMC3398792
Record Number:CaltechAUTHORS:20160818-105215538
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160818-105215538
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69759
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:18 Aug 2016 19:06
Last Modified:03 Oct 2019 10:25

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