A Caltech Library Service

Label-free oxygen-metabolic photoacoustic microscopy in vivo

Yao, Junjie and Maslov, Konstantin I. and Zhang, Yu and Xia, Younan and Wang, Lihong V. (2011) Label-free oxygen-metabolic photoacoustic microscopy in vivo. Journal of Biomedical Optics, 16 (7). Art. No. 076003. ISSN 1083-3668. PMCID PMC3144973.

[img] PDF - Published Version
See Usage Policy.


Use this Persistent URL to link to this item:


Almost all diseases, especially cancer and diabetes, manifest abnormal oxygen metabolism. Accurately measuring the metabolic rate of oxygen (MRO_2) can be helpful for fundamental pathophysiological studies, and even early diagnosis and treatment of disease. Current techniques either lack high resolution or rely on exogenous contrast. Here, we propose label-free metabolic photoacoustic microscopy (mPAM) with small vessel resolution to noninvasively quantify MRO_2 in vivo in absolute units. mPAM is the unique modality for simultaneously imaging all five anatomical, chemical, and fluid-dynamic parameters required for such quantification: tissue volume, vessel cross-section, concentration of hemoglobin, oxygen saturation of hemoglobin, and blood flow speed. Hyperthermia, cryotherapy, melanoma, and glioblastoma were longitudinally imaged in vivo. Counterintuitively, increased MRO_2 does not necessarily cause hypoxia or increase oxygen extraction. In fact, early-stage cancer was found to be hyperoxic despite hypermetabolism.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Maslov, Konstantin I.0000-0003-3408-8840
Zhang, Yu0000-0001-8938-1927
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2011 SPIE. Paper 11075R received Feb. 17, 2011; revised manuscript received Apr. 12, 2011; accepted for publication May 5, 2011; published online Jul. 1, 2011. The authors thank Christopher Favazza, Kim Chulhong, Song Hu, Lidai Wang, Dakang Yao, and Arie Krumholz for helpful discussions; Li Li for experimental assistance; and Professor James Ballard for manuscript editing. This research was supported by the National Institutes of Health Grants Nos. R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 EB010049, and 5P60 DK02057933.
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
NIHR01 EB010049
NIH5P60 DK02057933
Subject Keywords:metabolic rate of oxygen; photoacoustic imaging; hyperthermia; cryotherapy; tumor hypermetabolism; tumor hyperoxia
Issue or Number:7
PubMed Central ID:PMC3144973
Record Number:CaltechAUTHORS:20160819-133957547
Persistent URL:
Official Citation:Yao J, Maslov KI, Zhang Y, Xia Y, Wang LV; Label-free oxygen-metabolic photoacoustic microscopy in vivo. J. Biomed. Opt. 0001;16(7):076003-076003-11. doi:10.1117/1.3594786
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69794
Deposited By: Tony Diaz
Deposited On:22 Aug 2016 23:51
Last Modified:09 Mar 2020 13:19

Repository Staff Only: item control page