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Noninvasive Photoacoustic and Fluorescence Sentinel Lymph Node Identification using Dye-Loaded Perfluorocarbon Nanoparticles

Akers, Walter J. and Kim, Chulhong and Berezin, Mikhail and Guo, Kevin and Fuhrhop, Ralph and Lanza, Gregory M. and Fischer, Georg M. and Daltrozzo, Ewald and Zumbusch, Andreas and Cai, Xin and Wang, Lihong V. and Achilefu, Samuel (2011) Noninvasive Photoacoustic and Fluorescence Sentinel Lymph Node Identification using Dye-Loaded Perfluorocarbon Nanoparticles. ACS Nano, 5 (1). pp. 173-182. ISSN 1936-0851. PMCID PMC3026895.

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The contrast mechanisms used for photoacoustic tomography (PAT) and fluorescence imaging differ in subtle, but significant, ways. The design of contrast agents for each or both modalities requires an understanding of the spectral characteristics as well as intra- and intermolecular interactions that occur during formulation. We found that fluorescence quenching that occurs in the formulation of near-infrared (NIR) fluorescent dyes in nanoparticles results in enhanced contrast for PAT. The ability of the new PAT method to utilize strongly absorbing chromophores for signal generation allowed us to convert a highly fluorescent dye into an exceptionally high PA contrast material. Spectroscopic characterization of the developed NIR dye-loaded perfluorocarbon-based nanoparticles for combined fluorescence and PA imaging revealed distinct dye-dependent photophysical behavior. We demonstrate that the enhanced contrast allows detection of regional lymph nodes of rats in vivo with time-domain optical and photoacoustic imaging methods. The results further show that the use of fluorescence lifetime imaging, which is less dependent on fluorescence intensity, provides a strategic approach to bridge the disparate contrast reporting mechanisms of fluorescence and PA imaging methods.

Item Type:Article
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URLURL TypeDescription Information CentralArticle
Wang, Lihong V.0000-0001-9783-4383
Alternate Title:Non-invasive Photoacoustic and Fluorescence Sentinel Lymph Node Identification using Dye-loaded Perfluorocarbon Nanoparticles
Additional Information:© 2011 American Chemical Society. Received for review September 3, 2010; and accepted December 07, 2010. Published online December 20, 2010. These studies were funded, in part, by grants from the NIH (R01 EB008111 and R01 EB008458 to S.A. and R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 EB010049, and 5P60 DK02057933 to L.V.W). L.V.W. has a financial interest in Microphotoacoustics, Inc. and in Endra, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHR01 EB008111
NIHR01 EB008458
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
NIHR01 EB010049
NIH5P60 DK02057933
Subject Keywords:biopsy; cancer; metastasis; optical; perfluorocarbon; prognosis
Issue or Number:1
PubMed Central ID:PMC3026895
Record Number:CaltechAUTHORS:20160822-160844254
Persistent URL:
Official Citation:Noninvasive Photoacoustic and Fluorescence Sentinel Lymph Node Identification using Dye-Loaded Perfluorocarbon Nanoparticles Walter J. Akers, Chulhong Kim, Mikhail Berezin, Kevin Guo, Ralph Fuhrhop, Gregory M. Lanza, Georg M. Fischer, Ewald Daltrozzo, Andreas Zumbusch, Xin Cai, Lihong V. Wang, and Samuel Achilefu ACS Nano 2011 5 (1), 173-182 DOI: 10.1021/nn102274q
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69821
Deposited By: Tony Diaz
Deposited On:22 Aug 2016 23:30
Last Modified:03 Oct 2019 10:25

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