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Real-time four-dimensional optical-resolution photoacoustic microscopy with Au nanoparticle-assisted subdiffraction-limit resolution

Rao, Bin and Maslov, Konstantin and Danielli, Amos and Chen, Ruimin and Shung, K. Kirk and Zhou, Qifa and Wang, Lihong V. (2011) Real-time four-dimensional optical-resolution photoacoustic microscopy with Au nanoparticle-assisted subdiffraction-limit resolution. Optics Letters, 36 (7). pp. 1137-1139. ISSN 0146-9592. PMCID PMC3129741. https://resolver.caltech.edu/CaltechAUTHORS:20160829-133426861

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Abstract

Photoacoustic microscopy (PAM) offers label-free, optical absorption contrast. A high-speed, high-resolution PAM system in an inverted microscope configuration with a laser pulse repetition rate of 100,000 Hz and a stationary ultrasonic transducer was built. Four-dimensional in vivo imaging of microcirculation in mouse skin was achieved at 18 three-dimensional volumes per second with repeated two-dimensional (2D) raster scans of 100 by 50 points. The corresponding 2D B-scan (50 A-lines) frame rate was 1800 Hz, and the one-dimensional A-scan rate was 90,000 Hz. The lateral resolution is 0.23±0.03 μm for Au nanowire imaging, which is 2.0 times below the diffraction limit.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1364/OL.36.001137DOIArticle
https://www.osapublishing.org/ol/abstract.cfm?uri=ol-36-7-1137PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3129741PubMed CentralArticle
ORCID:
AuthorORCID
Rao, Bin0000-0001-6494-3110
Maslov, Konstantin0000-0003-3408-8840
Chen, Ruimin0000-0002-5338-359X
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2011 Optical Society of America. Received January 19, 2011; revised February 23, 2011; accepted February 25, 2011; posted March 3, 2011 (Doc. ID 140483); published March 23, 2011. We gratefully thank the National Institutes of Health (NIH) for supporting grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, and P41-EB2182. Help from D. Yao in preparing samples is appreciated. L. Wang has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
NIHR01 CA157277
NIHP41-EB2182
Issue or Number:7
PubMed Central ID:PMC3129741
Record Number:CaltechAUTHORS:20160829-133426861
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160829-133426861
Official Citation:B. Rao, K. Maslov, A. Danielli, R. Chen, K. Shung, Q. Zhou, and L. Wang, "Real-time four-dimensional optical-resolution photoacoustic microscopy with Au nanoparticle-assisted subdiffraction-limit resolution," Opt. Lett. 36, 1137-1139 (2011)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69991
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:29 Aug 2016 21:34
Last Modified:09 Mar 2020 13:19

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