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Calibration-free absolute quantification of optical absorption coefficients using acoustic spectra in 3D photoacoustic microscopy of biological tissue

Guo, Zijian and Hu, Song and Wang, Lihong V. (2010) Calibration-free absolute quantification of optical absorption coefficients using acoustic spectra in 3D photoacoustic microscopy of biological tissue. Optics Letters, 35 (12). pp. 2067-2069. ISSN 0146-9592. PMCID PMC3071532. http://resolver.caltech.edu/CaltechAUTHORS:20160830-083014111

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Abstract

Optical absorption is closely associated with many physiological important parameters, such as the concentration and oxygen saturation of hemoglobin, and it can be used to quantify the concentrations of nonfluorescent molecules. We propose a method to use acoustic spectra of photoacoustic signals to quantify the absolute optical absorption. This method is self-calibrating and thus insensitive to variations in the optical fluence. Factors such as system bandwidth and acoustic attenuation can affect the quantification but can be canceled by dividing the acoustic spectra measured at two optical wavelengths. Using optical-resolution photoacoustic microscopy, we quantified the absolute optical absorption of black ink samples with various concentrations. We also quantified both the concentration and oxygen saturation of hemoglobin in a live mouse in absolute units.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1364/OL.35.002067DOIArticle
https://www.osapublishing.org/ol/abstract.cfm?uri=ol-35-12-2067PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3071532PubMed CentralArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2010 Optical Society of America. Received February 9, 2010; revised May 14, 2010; accepted May 9, 2010; posted June 1, 2010 (Doc. ID 123979); published June 10, 2010. This work was sponsored in part by National Institutes of Health (NIH) grants R01 EB000712, R01 EB008085, R01 CA113453901, U54 CA136398, and 5P60 DK02057933. L. W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA113453901
NIHU54 CA136398
NIH5P60 DK02057933
Classification Code:OCIS codes: 170.3880, 170.5120, 300.1030, 170.1460
PubMed Central ID:PMC3071532
Record Number:CaltechAUTHORS:20160830-083014111
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160830-083014111
Official Citation:Z. Guo, S. Hu, and L. Wang, "Calibration-free absolute quantification of optical absorption coefficients using acoustic spectra in 3D photoacoustic microscopy of biological tissue," Opt. Lett. 35, 2067-2069 (2010)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:70015
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:31 Aug 2016 04:36
Last Modified:31 Aug 2016 04:36

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