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Integrated Photoacoustic and Fluorescence Confocal Microscopy

Wang, Yu and Maslov, Konstantin and Kim, Chulhong and Hu, Song and Wang, Lihong V. (2010) Integrated Photoacoustic and Fluorescence Confocal Microscopy. IEEE Transactions on Biomedical Engineering, 57 (10). pp. 2576-2578. ISSN 0018-9294. PMCID PMC2970769.

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We have developed a dual-modality imaging system by integrating optical-resolution photoacoustic microscopy and fluorescence confocal microscopy to provide optical absorption and fluorescence contrasts simultaneously. By sharing the same laser source and objective lens, intrinsically registered photoacoustic and fluorescence images are acquired in a single scan. The micrometer resolution allows imaging of both blood and lymphatic vessels down to the capillary level. Simultaneous photoacoustic angiography and fluorescence lymphangiography were demonstrated, presenting more information to study tumor angiogenesis, vasculature, and microenvironments in vivo.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Wang, Yu0000-0003-3589-9274
Maslov, Konstantin0000-0003-3408-8840
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2010 IEEE. Manuscript received May 1, 2010; revised June 11, 2010; accepted June 17, 2010. Date of publication July 15, 2010; date of current version September 15, 2010. This work was supported in part by the National Institutes of Health under Grant R01 EB000712, Grant R01 EB008085, Grant R01 CA113453901, Grant U54 CA136398, and Grant 5P60 DK02057933. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA113453901
NIHU54 CA136398
NIH5P60 DK02057933
Subject Keywords:Angiography, fluorescence confocal microscopy (FCM), lymphangiography, multimodal imaging, photoacoustic microscopy (PAM)
Issue or Number:10
PubMed Central ID:PMC2970769
Record Number:CaltechAUTHORS:20160830-104712331
Persistent URL:
Official Citation:Y. Wang, K. Maslov, C. Kim, S. Hu and L. V. Wang, "Integrated Photoacoustic and Fluorescence Confocal Microscopy," in IEEE Transactions on Biomedical Engineering, vol. 57, no. 10, pp. 2576-2578, Oct. 2010. doi: 10.1109/TBME.2010.2059026 URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:70035
Deposited By: Tony Diaz
Deposited On:31 Aug 2016 04:11
Last Modified:09 Mar 2020 13:19

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