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Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholera and are stabilized by a double signaling domain receptor DosM

Briegel, Ariane and Ortega, Davi R. and Mann, Petra and Kjær, Andreas and Ringgaard, Simon and Jensen, Grant J. (2016) Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholera and are stabilized by a double signaling domain receptor DosM. Proceedings of the National Academy of Sciences of the United States of America, 113 (37). pp. 10412-10417. ISSN 0027-8424. PMCID PMC5027440. http://resolver.caltech.edu/CaltechAUTHORS:20160831-084712382

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Abstract

Nearly all motile bacterial cells use a highly sensitive and adaptable sensory system to detect changes in nutrient concentrations in the environment and guide their movements toward attractants and away from repellents. The best-studied bacterial chemoreceptor arrays are membrane-bound. Many motile bacteria contain one or more additional, sometimes purely cytoplasmic, chemoreceptor systems. Vibrio cholerae contains three chemotaxis clusters (I, II, and III). Here, using electron cryotomography, we explore V. cholerae’s cytoplasmic chemoreceptor array and establish that it is formed by proteins from cluster I. We further identify a chemoreceptor with an unusual domain architecture, DosM, which is essential for formation of the cytoplasmic arrays. DosM contains two signaling domains and spans the two-layered cytoplasmic arrays. Finally, we present evidence suggesting that this type of receptor is important for the structural stability of the cytoplasmic array.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.1604693113DOIArticle
http://www.pnas.org/content/113/37/10412PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027440/PubMed CentralArticle
ORCID:
AuthorORCID
Briegel, Ariane0000-0003-3733-3725
Ortega, Davi R.0000-0002-8344-2335
Kjær, Andreas0000-0002-0096-5764
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2016 National Academy of Sciences. Edited by Caroline S. Harwood, University of Washington, Seattle, WA, and approved July 19, 2016 (received for review March 22, 2016). Published online before print August 29, 2016. We thank Drs. Zhiheng Yu, Jason de la Cruz, Chuan Hong, and Rick Huang for microscopy support at the Howard Hughes Medical Institute Janelia Research Campus. This work was supported by National Institute of General Medical Sciences Grant GM101425 (to G.J.J.) and by the Max Planck Society (S.R.). Author contributions: A.B., D.R.O., S.R., and G.J.J. designed research; A.B., D.R.O., P.M., and A.K. performed research; A.B., D.R.O., P.M., A.K., and S.R. analyzed data; and A.B., D.R.O., S.R., and G.J.J. wrote the paper. The authors declare no conflict of interest. This article is a PNAS Direct Submission. Data deposition: The electron cryotomography subtomogram average density map reported in this paper has been deposited in the Electron Microscopy Data Bank, www.emdatabank.org (accession no. EMD-3398).
Funders:
Funding AgencyGrant Number
NIHGM101425
Max Planck SocietyUNSPECIFIED
Subject Keywords:chemotaxis; chemoreceptor arrays; Vibrio cholerae; electron cryotomography; microscopy
PubMed Central ID:PMC5027440
Record Number:CaltechAUTHORS:20160831-084712382
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160831-084712382
Official Citation:Ariane Briegel, Davi R. Ortega, Petra Mann, Andreas Kjær, Simon Ringgaard, and Grant J. Jensen Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholerae and are stabilized by a double signaling domain receptor DosM PNAS 2016 113 (37) 10412-10417; published ahead of print August 29, 2016, doi:10.1073/pnas.1604693113
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:70053
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:31 Aug 2016 16:36
Last Modified:23 Apr 2019 22:02

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