Briegel, Ariane and Ortega, Davi R. and Mann, Petra and Kjær, Andreas and Ringgaard, Simon and Jensen, Grant J. (2016) Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholera and are stabilized by a double signaling domain receptor DosM. Proceedings of the National Academy of Sciences of the United States of America, 113 (37). pp. 10412-10417. ISSN 0027-8424. PMCID PMC5027440. doi:10.1073/pnas.1604693113. https://resolver.caltech.edu/CaltechAUTHORS:20160831-084712382
![]() |
PDF
- Published Version
See Usage Policy. 1MB |
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160831-084712382
Abstract
Nearly all motile bacterial cells use a highly sensitive and adaptable sensory system to detect changes in nutrient concentrations in the environment and guide their movements toward attractants and away from repellents. The best-studied bacterial chemoreceptor arrays are membrane-bound. Many motile bacteria contain one or more additional, sometimes purely cytoplasmic, chemoreceptor systems. Vibrio cholerae contains three chemotaxis clusters (I, II, and III). Here, using electron cryotomography, we explore V. cholerae’s cytoplasmic chemoreceptor array and establish that it is formed by proteins from cluster I. We further identify a chemoreceptor with an unusual domain architecture, DosM, which is essential for formation of the cytoplasmic arrays. DosM contains two signaling domains and spans the two-layered cytoplasmic arrays. Finally, we present evidence suggesting that this type of receptor is important for the structural stability of the cytoplasmic array.
Item Type: | Article | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Related URLs: |
| ||||||||||
ORCID: |
| ||||||||||
Additional Information: | © 2016 National Academy of Sciences. Edited by Caroline S. Harwood, University of Washington, Seattle, WA, and approved July 19, 2016 (received for review March 22, 2016). Published online before print August 29, 2016. We thank Drs. Zhiheng Yu, Jason de la Cruz, Chuan Hong, and Rick Huang for microscopy support at the Howard Hughes Medical Institute Janelia Research Campus. This work was supported by National Institute of General Medical Sciences Grant GM101425 (to G.J.J.) and by the Max Planck Society (S.R.). Author contributions: A.B., D.R.O., S.R., and G.J.J. designed research; A.B., D.R.O., P.M., and A.K. performed research; A.B., D.R.O., P.M., A.K., and S.R. analyzed data; and A.B., D.R.O., S.R., and G.J.J. wrote the paper. The authors declare no conflict of interest. This article is a PNAS Direct Submission. Data deposition: The electron cryotomography subtomogram average density map reported in this paper has been deposited in the Electron Microscopy Data Bank, www.emdatabank.org (accession no. EMD-3398). | ||||||||||
Funders: |
| ||||||||||
Subject Keywords: | chemotaxis; chemoreceptor arrays; Vibrio cholerae; electron cryotomography; microscopy | ||||||||||
Issue or Number: | 37 | ||||||||||
PubMed Central ID: | PMC5027440 | ||||||||||
DOI: | 10.1073/pnas.1604693113 | ||||||||||
Record Number: | CaltechAUTHORS:20160831-084712382 | ||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160831-084712382 | ||||||||||
Official Citation: | Ariane Briegel, Davi R. Ortega, Petra Mann, Andreas Kjær, Simon Ringgaard, and Grant J. Jensen Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholerae and are stabilized by a double signaling domain receptor DosM PNAS 2016 113 (37) 10412-10417; published ahead of print August 29, 2016, doi:10.1073/pnas.1604693113 | ||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
ID Code: | 70053 | ||||||||||
Collection: | CaltechAUTHORS | ||||||||||
Deposited By: | Tony Diaz | ||||||||||
Deposited On: | 31 Aug 2016 16:36 | ||||||||||
Last Modified: | 19 Apr 2022 17:18 |
Repository Staff Only: item control page