A Caltech Library Service

Sentinel Lymph Nodes in the Rat: Noninvasive Photoacoustic and US Imaging with a Clinical US System

Erpelding, Todd N. and Kim, Chulhong and Pramanik, Manojit and Jankovic, Ladislav and Maslov, Konstantin and Guo, Zijian and Margenthaler, Julie A. and Pashley, Michael D. and Wang, Lihong V. (2010) Sentinel Lymph Nodes in the Rat: Noninvasive Photoacoustic and US Imaging with a Clinical US System. Radiology, 256 (1). pp. 102-110. ISSN 0033-8419. PMCID PMC2897692.

[img] PDF - Published Version
Creative Commons Attribution Non-commercial No Derivatives.


Use this Persistent URL to link to this item:


Purpose: To evaluate in vivo sentinel lymph node (SLN) mapping by using photoacoustic and ultrasonographic (US) imaging with a modified clinical US imaging system. Materials and Methods: Animal protocols were approved by the Animal Studies Committee. Methylene blue dye accumulation in axillary lymph nodes of seven healthy Sprague-Dawley rats was imaged by using a photoacoustic imaging system adapted from a clinical US imaging system. To investigate clinical translation, the imaging depth was extended up to 2.5 cm by adding chicken or turkey breast on top of the rat skin surface. Three-dimensional photoacoustic images were acquired by mechanically scanning the US transducer and light delivery fiber bundle along the elevational direction. Results: Photoacoustic images of rat SLNs clearly help visualization of methylene blue accumulation, whereas coregistered photoacoustic/US images depict lymph node positions relative to surrounding anatomy. Twenty minutes following methylene blue injection, photoacoustic signals from SLN regions increased nearly 33-fold from baseline signals in preinjection images, and mean contrast between SLNs and background tissue was 76.0 ± 23.7 (standard deviation). Methylene blue accumulation in SLNs was confirmed photoacoustically by using the optical absorption spectrum of the dye. Three-dimensional photoacoustic images demonstrate dynamic accumulation of methylene blue in SLNs after traveling through lymph vessels. Conclusion: In vivo photoacoustic and US mapping of SLNs was successfully demonstrated with a modified clinical US scanner. These results raise confidence that photoacoustic and US imaging can be used clinically for accurate, noninvasive imaging of SLNs for axillary lymph node staging in breast cancer patients.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Maslov, Konstantin0000-0003-3408-8840
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2010 Radiological Society of North America. Green OA works will be published under a Creative Commons Attribution-NonCommerical-NoDerivatives 4.0 International License (CC BY-NC-ND), which allows users to share the authors’ work as long as appropriate credit is given to the creator of the work; the material is not used for commercial purposes; and the material is not remixed, transformed, or built upon by the user. We thank John Dean, MS, Viktor Gornstein, MS, and Adam Schleicher, BS (Philips Research North America, Briarcliff Manor, NY), for modifying the US imaging system used for photoacoustic imaging and Feng Gao, PhD (Washington University School of Medicine, St Louis, Mo), for assistance with statistical analysis. This research was supported by the National Institutes of Health (grants U54 CA136398, R01 EB000712, R01 NS46214, and R01 EB008085). Author contributions: Guarantors of integrity of entire study, T.N.E., C.K., M.P., L.J., M.D.P., L.V.W.; study concepts/study design or data acquisition or data analysis/interpretation, all authors; manuscript drafting or manuscript revision for important intellectual content, all authors; approval of fi nal version of submitted manuscript, all authors; literature research, T.N.E., C.K., M.P., L.J., J.A.M., L.V.W.; clinical studies, C.K., J.A.M.; experimental studies, T.N.E., C.K., M.P., L.J., K.M., Z.G., L.V.W.; statistical analysis, T.N.E., C.K., L.V.W.; and manuscript editing, T.N.E., C.K., M.P., L.J., J.A.M., M.D.P., L.V.W.
Funding AgencyGrant Number
NIHU54 CA136398
NIHR01 EB000712
NIHR01 NS46214
NIHR01 EB008085
Issue or Number:1
PubMed Central ID:PMC2897692
Record Number:CaltechAUTHORS:20160908-153729231
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:70215
Deposited By: Tony Diaz
Deposited On:09 Sep 2016 18:33
Last Modified:03 Mar 2020 13:01

Repository Staff Only: item control page