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Rhodium-conjugate fluorescent probes for diagnostic of mismatched DNA

Nano, Adela and Barton, Jacqueline (2016) Rhodium-conjugate fluorescent probes for diagnostic of mismatched DNA. In: 252nd American Chemical Society National Meeting & Exposition, 21-25 August 2016, Philadelphia, PA.

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In healthy cells, DNA base pair mismatches, as well as some other base pair anomalies derived from DNA base damage, are cor. through the mismatch repair (MMR) machinery. If left unrepaired, mismatches cause mutations, some of which promote cancerous transformations. Currently, there is a lack of effective mol. sensors for early cancer diagnosis. Therefore, developing mol. probes capable of detecting and signaling DNA base mismatches directly would be a powerful tool to this end. Previous studies have shown that rhodium metalloinsertors bind mismatches in double-strained DNA with high specificity. Nevertheless, these rhodium complexes show poor luminescence properties. Here we describe the design and study of rhodium conjugates tethered to environmentally sensitive fluorophore scaffolds. The fluorophore of choice is a cyanine dye where fluorescence emission strongly depends on its cis-/transphotoisomerization through excitation under appropriate wavelengths; photoisomerization, however, appears to be inhibited for DNA-binding by cyanine, leading to a signal-on emission. In our luminescent switches, the cyanine was covalently attached to a rhodium(III) metalloinsertor complex through a linear spacer i.e. polyamine or polyethylene glycol chain. The spectroscopic measurements in the presence of well matched and mismatched DNA support the notion that our systems are interesting probes for mismatch detection and signaling.

Item Type:Conference or Workshop Item (Paper)
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URLURL TypeDescription Website
Nano, Adela0000-0002-1984-5770
Barton, Jacqueline0000-0001-9883-1600
Additional Information:© 2016 American Chemical Society.
Record Number:CaltechAUTHORS:20160913-160418397
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:70321
Deposited By: Tony Diaz
Deposited On:29 Sep 2016 23:54
Last Modified:03 Oct 2019 10:29

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