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Synthesis and characterization of biologically stable, doped LaF_3 nanoparticles co-conjugated to PEG and photosensitizers

Bekah, Devesh and Cooper, Daniel and Kudinov, Konstantin and Hill, Colin and Seuntjens, Jan and Bradforth, Stephen and Nadeau, Jay (2016) Synthesis and characterization of biologically stable, doped LaF_3 nanoparticles co-conjugated to PEG and photosensitizers. Journal of Photochemistry and Photobiology A: Chemistry, 329 . pp. 26-34. ISSN 1010-6030. https://resolver.caltech.edu/CaltechAUTHORS:20160915-102007190

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Abstract

There has been significant recent interest in combining radiation therapy and photodynamic therapy for cancer treatment. One proposed method involves conjugating photosensitizers (PS) to scintillating nanoparticles. However, the production of nanoscintillators that are stable in biological media is problematic since many of the commonly used compositions chelate phosphate. In this paper, the problem was solved by first synthesizing LaF3 nanoparticles, followed by surface modification with alendronate, which serves as a stable anchor for conjugation of molecules of interest. The nanoparticles had an average diameter of 4 nm, and were doped with either Ce^(3+), Tb^(3+), or both. In every case, a LaF3 shell was added. In subsequent steps, poly(ethylene) glycol (PEG) and photosensitizers (either chlorin e6 or rose bengal) were covalently linked to the alendronate ligand via an amide bond. This new formulation is stable in several solutions and buffers used commonly in the laboratory for cell culture. Moreover, cellular uptake and lack of toxicity up to a concentration of 50 μM were observed in B16 murine melanoma cells. Energy transfer between the doped nanoparticle core and the conjugated photosensitizers was demonstrated using UV excitation, and scintillation was demonstrated using x-rays.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.jphotochem.2016.06.008DOIArticle
http://www.sciencedirect.com/science/article/pii/S1010603016300430PublisherArticle
ORCID:
AuthorORCID
Nadeau, Jay0000-0001-5258-0076
Alternate Title:Synthesis and characterization of biologically stable, doped LaF3 nanoparticles co-conjugated to PEG and photosensitizers
Additional Information:© 2016 Elsevier B.V. Received 21 January 2016, Revised 7 June 2016, Accepted 10 June 2016, Available online 18 June 2016.
Subject Keywords:Radiotherapy; Photodynamic therapy; Lanthanum fluoride; Lanthanide; Nanoscintillator; PEG; Rose bengal; Chlorin e6
Record Number:CaltechAUTHORS:20160915-102007190
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160915-102007190
Official Citation:Devesh Bekah, Daniel Cooper, Konstantin Kudinov, Colin Hill, Jan Seuntjens, Stephen Bradforth, Jay Nadeau, Synthesis and characterization of biologically stable, doped LaF3 nanoparticles co-conjugated to PEG and photosensitizers, Journal of Photochemistry and Photobiology A: Chemistry, Volume 329, 1 October 2016, Pages 26-34, ISSN 1010-6030, http://dx.doi.org/10.1016/j.jphotochem.2016.06.008. (http://www.sciencedirect.com/science/article/pii/S1010603016300430)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:70370
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Sep 2016 17:18
Last Modified:03 Oct 2019 10:30

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