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Wide-field Fourier ptychographic microscopy using laser illumination source

Chung, Jaebum and Lu, Hangwen and Ou, Xiaoze and Zhou, Haojiang and Yang, Changhuei (2016) Wide-field Fourier ptychographic microscopy using laser illumination source. Biomedical Optics Express, 7 (11). pp. 4787-4802. ISSN 2156-7085. PMCID PMC5119616.

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Fourier ptychographic (FP) microscopy is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method’s demand for multiple images drives the need for a brighter illumination scheme and a high-frame-rate camera for a faster acquisition. We report the use of a guided laser beam as an illumination source for an FP microscope. It uses a mirror array and a 2-dimensional scanning Galvo mirror system to provide a sample with plane-wave illuminations at diverse incidence angles. The use of a laser presents speckles in the image capturing process due to reflections between glass surfaces in the system. They appear as slowly varying background fluctuations in the final reconstructed image. We are able to mitigate these artifacts by including a phase image obtained by differential phase contrast (DPC) deconvolution in the FP algorithm. We use a 1-Watt laser configured to provide a collimated beam with 150 mW of power and beam diameter of 1 cm to allow for the total capturing time of 0.96 seconds for 96 raw FPM input images in our system, with the camera sensor’s frame rate being the bottleneck for speed. We demonstrate a factor of 4 resolution improvement using a 0.1 NA objective lens over the full camera field-of-view of 2.7 mm by 1.5 mm.

Item Type:Article
Related URLs:
URLURL TypeDescription Paper CentralArticle
Chung, Jaebum0000-0003-3932-8428
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2016 Optical Society of America. Received 5 Aug 2016; revised 10 Oct 2016; accepted 25 Oct 2016; published 31 Oct 2016. Funding: National Institute of Health (NIH) Agency Award: R01 AI096226; Caltech Innovation Initiative (CII): 25570015. We thank Daniel Martin for fabricating the Siemens star targets, and Mooseok Jang and Haowen Ruan for helpful discussions.
Funding AgencyGrant Number
NIHR01 AI096226
Caltech Innovation Initiative (CI2)25570015
Issue or Number:11
Classification Code:OCIS codes: (180.0180) Microscopy; (070.0070) Fourier optics and signal processing; (110.1758) Computational imaging
PubMed Central ID:PMC5119616
Record Number:CaltechAUTHORS:20160919-122718840
Persistent URL:
Official Citation:Jaebum Chung, Hangwen Lu, Xiaoze Ou, Haojiang Zhou, and Changhuei Yang, "Wide-field Fourier ptychographic microscopy using laser illumination source," Biomed. Opt. Express 7, 4787-4802 (2016)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:70428
Deposited By: Tony Diaz
Deposited On:30 Sep 2016 17:25
Last Modified:09 Mar 2020 13:19

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