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Continuing Rearrangement of Immunoglobulin and T-Cell Receptor Genes in a Ha-Ras-Transformed Lymphoid Progenitor Cell Line

Alessandrini, Alessandro and Pierce, Jacalyn H. and Baltimore, David and Desiderio, Stephen V. (1987) Continuing Rearrangement of Immunoglobulin and T-Cell Receptor Genes in a Ha-Ras-Transformed Lymphoid Progenitor Cell Line. Proceedings of the National Academy of Sciences of the United States of America, 84 (7). pp. 1799-1803. ISSN 0027-8424. PMCID PMC304528. doi:10.1073/pnas.84.7.1799.

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The arrangement of immunoglobulin genes has been examined in a series of lymphoid cell lines transformed with the Harvey murine sarcoma virus. One cell line, HAFTL-1, expresses antigenic markers characteristic of B-lymphoid cells and undergoes frequent rearrangement at the JH locus (where J = joining and H = heavy chain) during propagation in culture. By molecular cloning and nucleotide sequence determination, these rearrangements were found to represent the earliest postulated step in heavy chain gene assembly: the joining of a diversity (D) segment to a JH segment. The HAFTL-1 cell line also undergoes infrequent Dβ-to-Jβ joining at the T-cell receptor β locus in culture. The observations presented here suggest that the HAFTL-1 cell line represents the early stage of B-cell differentiation at which immunoglobulin gene rearrangement is initiated.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1987 by the National Academy of Sciences Contributed by David Baltimore, November 19, 1986 We thank Karen Wolff for excellent technical assistance. A.A. is supported by National Institutes of Health Training Grant 2 T32 CA09139. S.V.D. is an Assistant Investigator of the Howard Hughes Medical Institute. The work was supported by the Howard Hughes Medical Institute and by National Cancer Institute Grant 5 P01 CA16519 (to S.V.D.) and American Cancer Society Grant IM-355 (to D.B.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
NIH Predoctoral Fellowship2 T32 CA09139
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIH5 P01 CA16519
American Cancer SocietyIM-355
Subject Keywords:DNA rearrangement; lymphocyte differentiation; retroviral transformation
Issue or Number:7
PubMed Central ID:PMC304528
Record Number:CaltechAUTHORS:ALEpnas87
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7049
Deposited By: Archive Administrator
Deposited On:06 Jan 2007
Last Modified:08 Nov 2021 20:39

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