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Gα16, a G Protein α Subunit Specifically Expressed in Hematopoietic Cells

Amatruda, Thomas T., III and Steele, David A. and Slepak, Vladlen Z. and Simon, Melvin I. (1991) Gα16, a G Protein α Subunit Specifically Expressed in Hematopoietic Cells. Proceedings of the National Academy of Sciences of the United States of America, 88 (13). pp. 5587-5591. ISSN 0027-8424. PMCID PMC51922. doi:10.1073/pnas.88.13.5587.

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Signal-transduction pathways mediated by guanine nucleotide-binding regulatory proteins (G proteins) determine many of the responses of hematopoietic cells. A recently identified gene encoding a G protein α subunit, Gα16, is specifically expressed in human cells of the hematopoietic lineage. The Gα16 cDNA encodes a protein with predicted Mr of 43,500, which resembles the Gq class of α subunits and does not include a pertussis toxin ADP-ribosylation site. In comparison with other G protein α subunits, the Gα16 predicted protein has distinctive amino acid sequences in the amino terminus, the region A guanine nucleotide-binding domain, and in the carboxyl-terminal third of the protein. Cell lines of myelomonocytic and T-cell phenotype express the Gα16 gene, but no expression is detectable in two B-cell lines or in nonhematopoietic cell lines. Gα16 gene expression is down-regulated in HL-60 cells induced to differentiate to neutrophils with dimethyl sulfoxide. Antisera generated from synthetic peptides that correspond to two regions of Gα16 specifically react with a protein of 42- to 43-kDa in bacterial strains that overexpress Gα16 and in HL-60 membranes. This protein is decreased in membranes from dimethyl sulfoxide-differentiated HL-60 cells and is not detectable in COS cell membranes. The restricted expression of this gene suggests that Gα16 regulates cell-type-specific signal-transduction pathways, which are not inhibited by pertussis toxin.

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Additional Information:© 1991 by National Academy of Sciences Contributed by Melvin I. Simon, March 8, 1991 We are grateful to J. Didsbury, Duke University Medical Center, for providing a cDNA library; to H.P. Koeffler and to M.C. Territo, University of California, Los Angeles, Department of Medicine, for providing cell lines; and to M. Linder, University of Texas Southwestern Medical Center, for providing plasmid NpT7-5. We thank T. Wilkie and M. Strathmann for communicating unpublished data, and we thank B. Birren, T. Wilkie, and N. Gautam for a critical review of the manuscript. This work was supported by a Clinical Investigator Award from the National Cancer Institute (to T.T.A.) and by grants from the National Institutes of Health to M.I.S. The sequence reported in this paper has been deposited in the GenBank data base (accession no. M63904). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
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National Cancer InstituteUNSPECIFIED
Subject Keywords:signal transduction
Issue or Number:13
PubMed Central ID:PMC51922
Record Number:CaltechAUTHORS:AMApnas91
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7050
Deposited By: Archive Administrator
Deposited On:06 Jan 2007
Last Modified:08 Nov 2021 20:39

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